Skip to main content
  • Research article
  • Open access
  • Published:

Wnt activity guides facial branchiomotor neuron migration, and involves the PCP pathway and JNK and ROCK kinases



Wnt proteins play roles in many biological processes, including axon guidance and cell migration. In the mammalian hindbrain, facial branchiomotor (FBM) neurons undergo a striking rostral to caudal migration, yet little is known of the underlying molecular mechanisms. In this study, we investigated a possible role of Wnts and the planar cell polarity (PCP) pathway in this process.


Here we demonstrate a novel role for Wnt proteins in guiding FBM neurons during their rostral to caudal migration in the hindbrain. We found that Wnt5a is expressed in a caudalhigh to rostrallow gradient in the hindbrain. Wnt-coated beads chemoattracted FBM neurons to ectopic positions in an explant migration assay. The rostrocaudal FBM migration was moderately perturbed in Wnt5a mutant embryos and severely disrupted in Frizzled3 mutant mouse embryos, and was aberrant following inhibition of Wnt function by secreted Frizzled-related proteins. We also show the involvement of the Wnt/PCP pathway in mammalian FBM neuron migration. Thus, mutations in two PCP genes, Vangl2 and Scribble, caused severe defects in FBM migration. Inhibition of JNK and ROCK kinases strongly and specifically reduced the FBM migration, as well as blocked the chemoattractant effects of ectopic Wnt proteins.


These results provide in vivo evidence that Wnts chemoattract mammalian FBM neurons and that Wnt5a is a candidate to mediate this process. Molecules of the PCP pathway and the JNK and ROCK kinases also play a role in the FBM migration and are likely mediators of Wnt signalling.


Neuronal migration is a fundamental feature of the developing nervous system [1]. A striking migration is undertaken by the facial branchiomotor neurons (FBMs) of the mammalian embryonic hindbrain. In the mouse embryo, FBM neurons differentiate within rhombomere 4 (r4), while facial visceral motor neurons differentiate in r5 [2, 3]. FBM neurons extend axons via the facial nerve (between embryonic day (E)10.5 and E13.5); concomitantly, neuronal somata migrate caudally from r4 to r6 commencing around E10.5, and finally migrate radially within r6 to form the facial motor nucleus [2, 4]. There is very little information about the molecules that drive FBM migration in the mouse embryo. Here we demonstrate for the first time a role for the Wnt/planar cell polarity (PCP) pathway in this process in mammals.

In vertebrates, the PCP pathway has been shown to function during the convergent extension movements of gastrulation and neurulation, and in hair cell polarisation (reviewed by [5, 6]). The 'non-canonical Wnts', including Wnt5a, Wnt7a and Wnt11 are implicated in these processes [710]. Although the evidence linking Wnts with the PCP pathway is controversial, PCP signalling operates via Frizzleds (Fzs), and the membrane protein VanGogh-like 2 (Vangl2), while the cytoplasmic protein Scribble1 (Scrb1; which has been linked to apico-basal polarity) may also play a role. These components activate Dishevelled, which activates RhoA/Rho kinase (ROCK), and/or Rac and c-Jun amino-terminal kinase (JNK), leading to phosphorylation of molecules involved in cytoskeletal dynamics [11, 12]. The Wnt/PCP molecule Vangl2 can bind to Dishevelled, and can signal via both ROCK and JNK [1316]. In addition, Wnt5a can activate JNK to influence convergent extension [17], and is required for the localisation of Vangl2 in cochlear hair cells [10]. Thus, it is plausible that in FBM migration, JNK and ROCK form part of a signalling cascade containing Wnt5a, Fz, Vangl2, and other PCP components. Other downstream kinases that might function in FBM migration include myosin light chain kinase (MLCK), which is phosphorylated by ROCK and is implicated in cell migration (reviewed by [18]).

While recent (mainly loss-of-function) studies suggest that the Wnt/PCP pathway functions in FBM migration in the zebrafish [1922], it is unclear which mechanisms operate in mammals (reviewed by [23]). In mice, there are few studies on the mechanisms of FBM migration, although vascular endothelial growth factor (VEGF) has been proposed as a chemoattractant [24]. In this paper, we demonstrate a role of Wnts, other Wnt/PCP components and their downstream signalling pathways in the migration of FBM mammalian neurons.

Materials and methods


For appropriate gestational ages, we counted 0.5 days post-coitus as the formation of a vaginal plug on the following morning after matings. CD1 females were used as the wild-type strain. Vangl2, Scribble, Fz3 and Wnt5a homozygous mutants were obtained and genotyped as previously described [10, 15, 21, 25, 26]. Fz3 mutants were kindly provided by Dr Jeremy Nathans.

Hindbrain explant cultures

Entire hindbrain explants were cultured on millipore filters (Costar, Fisher, Loughborough, Leicestershire, UK) as previously described [24] and were supplemented with glial cell line-derived neurotrophic factor (R&D systems, Abingdon, Oxfordshire, UK). For chemoattraction affinity bead experiments, Cibacron blue 3AG agarose beads (Sigma-Aldrich, St. Louis, MO, USA) were soaked in phosphate-buffered saline (PBS; control) or in 100 μg/ml of either Wnt5a, Wnt7a, Semaphorin 3A (Sema3A; R&D systems) or VEGF (Peprotech Inc., London, UK) at 4°C overnight, before implantation into hindbrain explants. For the inhibitor experiments, explants were treated with culture medium containing either 10 μM JNK inhibitor (SP 600125, Tocris Bioscience, Bristol UK), 10 μM ROCK inhibitor (Y27632, Calbiochem, UK) or 10 μM MLCK inhibitor (ML-7, Calbiochem). In some cases, inhibitors were applied to hindbrain explants containing Wnt beads to test whether chemoattraction by beads was blocked. For inhibition of Wnt function, a cocktail of secreted frizzed-related proteins (SFRPs; 1, 2 and 3; 500 ng/ml; R&D systems) was applied to explants in the culture medium. Although binding specificities of SFRPs are not completely characterised, SFRP 1 and 2 can bind Wnt5a (for example, see [27]). A combination of SFRPs was applied to block a spectrum of putative Wnt-mediated interactions. After 2 days, explants were fixed with 4% paraformaldehyde for 1 hour and processed for immunohistochemistry.

Immunohistochemistry and scoring of FBM migration in explants

After fixation, explants were washed several times with PBS containing 1% Triton (PBT) then blocked in PBT containing 10% sheep serum for 30 minutes. Subsequently, the explants were incubated overnight at 4°C with anti-Islet1/2 antibody (4D5; 1:200; Developmental Studies Hybridoma Bank, Iowa City, IA, USA), then washed with PBT for several hours, and afterwards incubated for 1 hour with Cy3 anti-mouse secondary antibody (1:800; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA). Finally, the explants were washed with PBT and mounted on slides using Fluor-Save (Calbiochem).

Each explant was scored blind using one of the two following scoring systems. For bead implantation chemoattraction assays, explants were scored as 'attraction' if FBM neurons were deflected from their normal trajectory or as 'no attraction' if the FBM migration stream was unperturbed. Explants containing beads plus inhibitors were scored in the same way, focussing on whether FBM cells were deflected from the migration path, rather than on the migration as a whole. For explants treated with inhibitors, explants were scored on a scale of 1–3, ranging from loss of migration (1) to intermediate loss of migration (2) to normal migration (3). As some neurons had already reached r5 in most explants at the time of culture, we scored loss of migration (1) as a failure of neurons to reach r6 and/or a reduction in the number of neurons that had reached r5. Intermediate loss of migration was scored if explants manifest a phenotype between normal and loss of migration, that is, some neurons having reached r6 but less than in the normal case. In some experiments using inhibitors, the dorsal migration of trigeminal motor neurons in r2/3 was also quantified on a 1–3 scale. For each treatment, the percentage of explants in each category was calculated and compared statistically between control and treated groups (χ-squared test).

Quantification of FBM migration in explants and in Wnt5a mutant embryos

For experiments with control (PBS-treated) beads, Wnt5a-treated beads alone or with JNK or ROCK inhibitors, migration was quantified using the Scion image (NIH image) programme. Confocal images were rendered in black and white and inverted so that black pixels represented migrating neurons. For explants containing beads, a box was drawn containing the beads themselves (located at the r3/4 boundary) and encompassing r4 ipsilateral to the beads up to the midpoint of the floor plate (Additional file 1A). The number of pixels within the box was counted and the mean number of pixels under each condition was presented graphically. For explants without beads and cultured as controls or with inhibitors, a box was drawn over r4 bilaterally and over r6 bilaterally, containing the FBM neuron migratory stream (Additional file 1E). The ratio of r6 pixels/r4 pixels gives an indication of how successfully migration has occurred. The mean ratio was calculated under each condition. For Wnt5a mutant embryos and wild-type littermates, the width of the FBM migratory stream was measured at a dorsoventral midpoint using the Scion image programme to quantify the defect. Statistical comparisons were done using a t-test.

In situ hybridisation and immunohistochemistry

In situ hybridisation on whole-mounts or on cryosections of mouse embryos was performed as previously described [28, 29]. Tissues were hybridised with specific digoxigenin-labeled probes for Islet-1 (gift from Professor A Simeone, SEMM, Naples, Italy), Wnt 5a, Wnt 7a, EphA4 (gift from Dr U Drescher, MRC Centre of Developmental Neurobiology, London, UK), or Slit1 (gift from Dr M Tessier-Lavigne, Genentech, San Francisco, USA).


Wnt 5a and Wnt7a attract FBM neurons in a migration assay

In the mouse embryo, FBM migration occurs between E10.5 and E14.5. FBM neurons are born in r4 from E9.5 onwards; and start migrating at E10.5, reaching r5 by E11.5 and r6 by E12.5 (Figure 1A–C), [2, 4]. In r6, neurons migrate laterally,, starting to form a nucleus by E12.5 (Figure 1C), with nucleus formation continuing until E14.5. The migration can be followed by in situ hybridisation for Islet-1, which is motor neuron-specific at r4-6 axial levels [30] (Figure 1D–F).

Figure 1
figure 1

Spatiotemporal pattern of facial branchiomotor (FBM) neuronal migration in vivo and in explants. (A-C) Schematic representations of mouse embryonic hindbrain at embryonic stages (E)10.5 (A), E11.5 (B) and E12.5 (C). FBM neurons are shown by black dots and migrate from rhombomere (r)4 (E10.5) to r6 (E12.5). Grey patches represent dorsal exit points of motor axons. (D-F) Islet-1 in situ hybridisation on flatmounted hindbrains showing FBM neurons migrating from r4 to r6 at E10.5 (D), E11.5 (E) and E12.5 (F). (G-I) Hindbrain explants cultured on filters and immunostained with anti-Islet 1/2 antibody. Time 0 h (G) represents the beginning of culture period when some FBM neurons have started to migrate into r5. Time 24 h (H) shows that more FBM neurons are present in r5 and that some have started to turn dorsally into r6. Time 48 h (I) shows FBM neurons that have reached their final destination in r6 to form a nucleus. TN, trigeminal motor nucleus. Scale bars: 500 μm in (D-F); 250 μm in (G-I).

In order to investigate the molecular mechanisms of FBM migration, we used a migration assay [24] in which E11.5 mouse hindbrains were isolated and cultured, flattened on filters, for 48 hours. Hindbrains were dissected out early on E11.5, as it was found that isolation of hindbrains on E10.5 led to poor tissue and motor neuron viability. Immunostaining of explants with anti-Islet-1/2 antibody at time 0 showed that most FBM neurons were located in r4, whereas a minority had migrated into r5 (Figure 1G). After 24 hours in vitro, many FBM neurons had reached r5 and some had reached r6 and started to turn laterally (Figure 1H). After 48 hours, FBM neurons had reached r6 and coalesced into a characteristic compact nucleus (Figure 1I), reflecting a similar or slightly later developmental stage than that observed using Islet-1 in situ hybridisation on E12.5 hindbrains (Figure 1F). Other branchiomotor neurons, such as those of the trigeminal nucleus, which undergo a lateral migration, were also visualised using Islet-1/2 immunostaining (data not shown).

We tested the effects of Wnt proteins on this migration pattern, using beads soaked in Wnt5a or Wnt7a protein (or PBS controls) in E11.5 hindbrain explants cultured for 48 hours. These are 'non-canonical' Wnts, which have been linked to convergent extension movements in fish and frogs (reviewed by [5, 31]). When PBS-soaked control beads were placed unilaterally in rostral r4, we found that the FBM migration resembled that in controls, that is, cells were not deflected from their normal migration route (Figure 2A, A'). However, placement of beads soaked in Wnt5a or Wnt7a protein led to a coalescence of FBM neurons around the beads, suggesting that there was a chemoattractant effect (Figure 2B, B'). FBM neurons in r4 and also in r5 migrated laterally, and in some cases cells from the contralateral side also moved across the midline towards the beads. Three-dimensional confocal images of these explants suggested that FBM neurons had collected around the beads. Explants containing either PBS-soaked or Wnt-soaked beads were then scored blind as to whether FBM neurons were deflected from their normal course ('attraction') or not ('no attraction'). Quantification and statistical analysis showed that this effect was significantly different from controls (Figure 2C). We also quantified migration in the presence of Wnt-coated and PBS control beads by pixel counting using the Scion image programme (see Materials and methods; Additional file 1A, B). This method also showed a significant difference between the two groups. In separate experiments, Wnt beads were also presented in r6/r7 to test whether they could attract FBM neurons caudally. An effect was detected, but showed somewhat less clearly that FBM neurons deviated from their pathway than that obtained from rostral placement of beads, possibly because of the proximity to the normal migration route/FBM nucleus (data not shown). However, our data are consistent with a role for Wnts in the caudal and lateral displacement of FBM neurons.

Figure 2
figure 2

Chemoattractant effect of Wnt proteins in hindbrain explants. (A-B') Embryonic stage (E)11.5 mouse hindbrain explants with Wnt-coated beads placed in a region of rhombomere (r)4 rostral and lateral to the facial branchiomotor migratory stream: (A) phosphate-buffered saline (PBS) control; (B) Wnt protein; (A', B') higher magnifications of regions in boxes in (A, B), respectively. White circles mark beads. Scale bars: 250 μm in (A, B); 125 μm in (A', B'). (C, D) Quantification of bead experiments, with proteins as indicated. (C) Wnt7a versus control p < 0.001, Wnt5a versus control p < 0.001 (indicated by asterisks), n = 25 explants in each group. (D) Vascular endothelial growth factor (VEGF) versus control p < 0.01 (positive control; indicated by single asterisk). Semaphorin 3A (Sema3A) versus control p > 0.05 (negative control; indicated by double asterisks), n = 10 explants in each group.

Previous studies have shown that FBM neuron migration is, in part, dependent on VEGF binding to its neuropilin-1 (Npn-1) receptor, whereas the alternative Npn-1 ligand Sema3A plays no role [24]. We therefore used VEGF and Sema3A-coated beads in this assay as positive and a negative controls, respectively. Our results confirmed that while VEGF acted as a chemoattractant, Sema3A had no effect (Figure 2D). These data show that Wnt5a and Wnt7a can chemoattract FBM neurons in hindbrain explants. In our assay, the magnitude of the effect is similar to that of VEGF.

Wnt5a and Wnt7a expression patterns in the hindbrain are consistent with a possible role as FBM guidance cues

In order to determine whether Wnt5a and/or Wnt7a might function as chemoattractants in vivo, we analysed their expression profiles in the mouse hindbrain. Whole-mount in situ hybridisation at E11.5 showed that in isolated neuroepithelial preparations, Wnt7a is expressed throughout the hindbrain, including a dorsal stripe and the floor plate (Figure 3A). Cryosections in situ-hybridised for Wnt7a mRNA showed that this expression was in the ventricular zone and generally in the neuroepithelium (Figure 3B–D). Intriguingly, whole-mount in situ hybridisation for Wnt5a showed a different pattern, with a caudalhigh to rostrallow gradient. This was a step gradient rather than a smooth gradient, with changes in expression level coinciding with rhombomere boundaries. There was little or no expression in r2/r3 and r4, apart from the floor plate, higher levels in r5/6 and then increased expression caudally, including a dorsal stripe (Figure 3E). Analysis of in situ-hybridised cryosections confirmed that Wnt5a forms a gradient in the hindbrain with higher levels caudally (Figure 3F–H). Taken together with the chemoattractant effect of Wnt protein in vitro, these expression patterns indicate that Wnt5a in particular could chemoattract FBM neurons along a rostrocaudal migration path in vivo, although Wnt7a could play an adjunct role. The rostrocaudal gradient of Wnt5a expression demonstrated here is consistent with previous data showing an attractant effect of r5/6 on FBM neurons in heterospecific mouse/chick grafting experiments [32].

Figure 3
figure 3

Expression patterns of Wnt 7A and Wnt 5A in the hindbrain. (A, E) mRNA in situ hybridisation for Wnt7A and Wnt5A on flat-mounted hindbrains of embryonic stage (E)11.5 mouse embryos, dissected free of all surrounding tissues. (B-D, F-H) Cryosections of E11.5 mouse embryo in situ hybridised for Wnt7A and Wnt5A genes, respectively. Rhombomere (r) levels are r4 (B, F), r5 (C, G) and r7 (D, H). Scale bars: 250 μm in (A, E); 500 μm in (B-D, F-H).

FBM migration is severely disrupted in Vangl2 and Scribble mutants

Since it is possible that in this system Wnts operate via, or co-operate with, the PCP signalling pathway, we next analysed loop-tail and circle-tail mice, which are mutant for the Vangl2 and Scribble genes, respectively [25, 33]. These mice manifest neural tube defects, including an open hindbrain. However, observation of flat-mounted preparations showed that normal rhombomere morphology was maintained. mRNA in situ hybridisation of mutant hindbrains for EphA4 and Krox-20 showed that these genes were expressed in r3 and r5, while Slit1 was expressed in the floor plate as previously reported [3436] (Additional file 2A, B, E, F; data not shown). We therefore compared the pattern of FBM neuronal migration using in situ hybridisation for Islet-1 on wild-type, homozygous and heterozygous Vangl2 and Scribble hindbrains at E11.5 and E13.5. We observed dramatic defects in FBM migration in the homozygous embryos of both lines compared to wild-type embryos. In wild-type embryo litter-mates at E11.5, there was a normal distribution of FBM neurons, many of which had reached r5 (Figures 1 and 4A). Some had started to migrate laterally in r6 (Figure 4A, C). By contrast, in Vangl2 and Scribble mutants at E11.5, all FBM neurons had failed to migrate caudally and were still located in r4 (Figure 4D, F, G).

Figure 4
figure 4

Analysis of Vangl2 and Scribble mutant phenotypes. (A, B, D, E, G, H, I-K) In situ hybridization with Islet-1 probe on flat-mount hindbrains showing facial branchiomotor (FBM) migration in wild-type embryos, and Vangl2 and Scribble heterozygotes and homozygotes. Embryonic stages (E) and probes are as indicated. Both homozygote mutants present a very dramatic failure of FBM migration (asterisks in (D, E, G, H)) with a population of motor neurons in the floor plate (FP, arrowheads). (J, K) In heterozygous Vangl2 and Scribble (Scrb) littermates at E13.5, a stream of cells arrest in the dorsal region of rhombomere (r)5 (arrowheads) compared to wild type (WT) (C, F) Transverse sections through the hindbrains of wild-type (C) and Vangl2-/- (F) hindbrains, immunostained with anti-Islet1/2 antibody to show FBM neurons migrating laterally in r6 (C) to form the FBM nucleus (asterisk) or remaining in r4 (asterisk in F). ( I). Scale bars: 250 μm in (A, B, D, E, G, H); 100 μm in (C, F); 125 μm in (I-K). TN, trigeminal motor nucleus.

By E13.5 in wild-type embryos (Figure 4B), FBM neurons had clustered in r6 while in both Vangl2 and Scribble mutants there was a highly abnormal distribution of FBM neurons, with the majority having migrated laterally to form an ectopic facial motor nucleus in dorsal r4 (Figure 4E, H). This migration mode is more characteristic of other populations of hindbrain branchiomotor neurons, for example, trigeminal [37]. Another subpopulation of FBM neurons was located in the centre of the floor plate in r4 (Figure 4E, H). Some heterozygotes of both lines appeared normal, but in a few cases at E13.5 there were FBM neurons in rostral r5 that had diverged from the migratory stream, and the facial motor nucleus appeared less compact compared with wild-type embryos, occupying part of r5 as well as r6 (Figure 4I–K). These observations suggest that the normal caudal migratory path of FBM neurons had been transformed into a lateral or medial migration route in the absence of either of these two PCP genes (Figure 4G–I). The aberrant lateral FBM migration is reminiscent of that seen in Hoxb1 mutants at early developmental stages (E11.5), in which mis-specification of the FBM population results in a 'default state' of lateral migration, characteristic of branchiomotor neurons at other axial levels [2, 38]. The aberrant medial migration route suggests that some FBM neurons might fail to express receptors for Netrin-1 or Slit, which are normally involved in repelling cranial motor neurons from the floor plate [36, 39]. In Vangl2 and Scribble homozygous embryos, the lateral migration of other branchiomotor neuron populations was normal, for example, trigeminal (Figure 4E, H), suggesting that these genes specifically direct the caudal FBM migration. mRNA in situ hybridisation for Wnt5a in the Vangl2 mutant background showed a normal distribution of transcript, excluding the possibility that the mutant phenotype could be caused by the absence or a change in the expression of Wnt5a (Additional file 2C, D).

Attenuation of Wnt signalling in vitro and in vivo disrupts FBM migration

SFRPs are known to interfere specifically with Wnt-Fz-dependent processes (reviewed by [40]) and can be used to establish an intrinsic function of Wnts. We applied a combination of SFRPs 1–3 to hindbrain explants at the beginning of the culture period. The majority of explants showed severe disruption of the caudal FBM migration, compared with control explants (Figure 5A, B). Explants were scored on a scale of 1–3, ranging from complete loss of migration (1) to intermediate loss of migration (2) to normal migration (3). A comparison of values in untreated versus SFRP-treated explants showed a significant shift in values towards a loss of migration, suggesting that SFRPs strongly inhibit this process (Figure 5C). A caveat to this interpretation may be that SFRPs can, under some circumstances, bind to Fzs and act as guidance cues [41]. However, we believe that, taken together with data from Wnt and Fz mutant embryos (see below), our results suggest that endogenous Wnt-Fz interactions drive FBM migration.

Figure 5
figure 5

Analysis of the effects of attenuation of Wnt signalling on facial branchiomotor (FBM) migration. (A, B) Control hindbrain explant (A) and secreted frizzed-related protein (SFRP)-treated explant (B) immunostained for Islet-1/2 after 48 hours in vitro. (C) Quantification of FBM migration in hindbrain explants treated with SFRPs. SFRP-treated versus control p < 0.01 (indicated by asterisk), n = 10 explants in each group. (D, E, G-M) Analysis of mutant phenotypes by in situ hybridisation with Islet-1 probe. (D, E, G-I) Flat-mounted hindbrains of embryonic stage (E)12.5 mouse embryos of Wnt5A+/+ (wild type) and homozygote mutant litter-mates Wnt5A-/- in which a stream of cells (arrowheads in (G-I)) is located in rhombomere (r)5 and does not reach their final position in r6 compared to wild type (D). (E, H) Higher magnifications of FBM neurons in wild type and homozygote mutant showing a decompacted nucleus in the latter. (F) Quantification of the migration defect by measurement of the width of the FBM migratory stream showing this is increased in Wnt5A mutants. N = 8; p < 0.05 (error bars = sems). (J, L) Flat-mounted hindbrains of E11.5 mouse embryos of Fz3+/+ (wild type) and its Fz3-/- litter-mate homozygote mutant in which a striking loss of migration of the FBM population can be observed. In the Fz3-/- mutant a much reduced stream of cells migrated caudally into r5 (asterisk) and there is aberrant dorsolateral migration of FBM neurons in r4 (arrowhead). (K, M) At E14.5, FBM neurons in homozygote Fz3-/- hindbrains (M) contain an FBM nucleus in r6 that is much smaller than in the wild-type (K) and there are ectopic dorsal nuclei in r4 and r5 (arrowheads). Brackets in (K, M) indicate the smaller size of the FBM nucleus in Fz3-/- mutant. Scale bars: in (B), 250 μm and applies to (A, B); in (G), 375 μm and applies to (D, G, I,); in (H), 125 μm and applies to (E, H); in (M), 375 μm and applies to (J, M). FN, facial nucleus; TN, trigeminal nucleus.

We also analysed Wnt7a and Wnt5a mutant mouse embryos, by performing mRNA in situ hybridisation for Islet-1 on E11.5 hindbrains. In Wnt7a mutant embryos, the distribution of FBM neurons closely resembled that in wild-type litter-mates, suggesting that there was no defect (data not shown). However, Wnt5a mutants showed a partial defect in the migration, with some divergent streams of migrating cells, resulting in a broader and less compact FBM nucleus extending into r5 as well as r6, suggesting that some of the FBM neurons had stalled (Figure 5D, E, G–I). This phenotype resembled the phenotype of heterozygous Vangl2 and Scribble mutant embryos (Figure 4J, K). Wnt5a heterozygous embryos showed no defects and were indistinguishable from wild-type embryos (Figure 5D; data not shown).

In order to quantify the Wnt5a mutant defect, we measured the width of the FBM migratory stream at a mid-point between the ventral edge of the migratory stream and the dorsal edge of the nucleus. This method revealed a significant increase in the width of the migratory stream in Wnt5a mutants compared with heterozygous embryos (Figure 5F), consistent with the decompaction of the nucleus observed. The phenotype that we observe in Wnt5a mutants suggests that Wnt5a plays a role in FBM migration in vivo, although we hypothesise that other Wnts might also play a role.

A candidate receptor to mediate the effect of Wnt in our system is Fz3, which is expressed in the mouse hindbrain at relevant developmental stages [40]. Fz3 is involved in hair cell polarity in the mouse and its homologue, Fz3a, is implicated in FBM migration in the zebrafish [21, 42, 43]. We investigated the migration pattern of FBM neurons in Fz3 mutants [44] by Islet-1 in situ hybridisation as above. We found that there were striking defects in FBM migration in E11.5 and E14.5 homozygous Fz3 mutants compared with their wild-type or heterozygous littermates. At E11.5 the majority of FBM neurons had failed to migrate caudally out of r4 and remained close to the midline (Figure 5L, compare with Figure 5J). A reduced number of FBM neurons could be observed migrating caudally, but many neurons also formed streams migrating laterally in r4 and r5. At E14.5 the FBM nucleus was well-formed in wild-type embryos and was located in r6 (Figure 5K). In Fz3 mutants, an FBM nucleus was present in r6, but it was strongly reduced in size (Figure 5M). The presence of ectopic nuclei in dorsal r4 and r5 suggests that these originated from the dorsally migrating FBM neurons observed at E11.5. Thus, in Fz3 mutants, FBM migration shows severe defects consistent with the involvement of Fz signalling in this system. Attenuation of this pathway leads to many FBM neurons following a lateral 'default' migration pathway, in the manner of other hindbrain branchiomotor neuron subpopulations.

JNK and ROCK kinases signal during FBM migration and are required for the attractant effects of Wnts

In order to dissect the signalling pathways involved in FBM migration, we applied pharmacological inhibitors of candidate molecules to the FBM migration assay. Inhibitors were applied at the beginning of the culture period and the distribution of Islet-1/2-positive FBM neurons was compared between inhibitor-treated explants and those treated with vehicle alone (DMSO controls). Migration was scored on a scale of 1–3 as for SFRP-treated explants. Explants treated with JNK inhibitor (SP600125) showed a dose-dependent disruption of migration, with 5 μM resulting in a moderate inhibition (57.2% of explants were grade 1 (loss of migration); data not shown) and 10 μM producing an effect in which FBM neurons stopped in r5 (68% of explants were scored as grade 1; Figure 6A–C). Inhibitors of ROCK (Y27632) or its downstream target MLCK (ML-7) both caused FBM migration defects, but in fewer cases, with 40–50% of explants showing grade 1 morphology (Figure 6D–I).

Figure 6
figure 6

Inhibition of facial branchiomotor (FBM) migration and of Wnt-mediated attraction. (A, D, G) Control explants cultured for 48 hours and immunostained with anti-Islet 1/2 antibody with FBM neurons located in their final position forming the facial motor nuclei in rhombomere (r)6. (B, E, H) Explants treated with c-Jun amino-terminal kinase (JNK) inhibitor (SP600125), Rho kinase (ROCK) inhibitor (Y27632) and myosin light chain kinase inhibitor (ML-7) as labelled. Scale bars: 250 μm (A, B, D, E, G, H). (C, F, I) Quantification of the effects of inhibitors on FBM migration in explants. The x-axis is the migration scale where 1 = loss of migration, 2 = intermediate loss of migration, 3 = normal migration. The y-axis is the percentage of explants in a particular category. Inhibitor-treated versus control p < 0.001 (indicated by asterisk). (J, K) Quantification of the effect of inhibitors on Wnt5A attractive beads. The attractive effect of Wnt5A ectopic beads is inhibited by adding inhibitors SP600125 (J) and Y27632 (K). Inhibitor-treated versus control p > 0.05 (indicated by asterisk). (C, F, I, J, K) N = 15–20 explants in each group.

To discover whether these inhibitors produced a general block of neuronal migration in the hindbrain, or had specific effects on the FBM caudal migration, we also assessed their effects on laterally migrating trigeminal (branchiomotor) neurons. JNK and ROCK inhibitors had modest effects in inhibiting lateral migration, though these effects were considerably less than those on FBM neurons' caudal migration (Additional file 3A, B). However, inhibition of MLCK produced effects that were more pronounced (45% of explants at grade 1) and equivalent to those on the FBM caudal migration (Additional file 3C). These data suggest that while MLCK plays a general role in cranial motor neuron migration, JNK and ROCK show specificity in functioning within the FBM caudal migration. As MLCK is a direct target of ROCK, this suggests that MLCK function in the dorsal migration might also be regulated by other upstream components. We also tested whether JNK and ROCK inhibitors blocked FBM neuron attraction by VEGF. Scoring of explants indicated that these inhibitors failed to block attraction by VEGF beads, indicating that JNK and ROCK are specific to Wnt-mediated FBM migration (Additional file 1C, D).

In view of the fact that Wnt5a is the most promising candidate to be an FBM chemoattractant in vivo, we next tested whether JNK or ROCK functioned downstream of Wnt5a, by applying inhibitors to explants containing implanted Wnt5a beads. The attractant effect of Wnt5a beads was specifically blocked by adding 10 μM of JNK inhibitor or of ROCK inhibitor (Figure 6J, K). This effect was further quantified by pixel counting (Materials and methods) of the number of FBM neurons in r4 adjacent to Wnt5a beads alone or in the presence of inhibitors. This showed a significant reduction in the number of FBM neurons adjacent to Wnt beads when explants were treated with inhibitors compared with those containing Wnt5a beads alone (Additional file 1B). This suggests that the effect of Wnt5a in FBM migration is mediated by the JNK and ROCK kinases.


We here propose that Wnt5a/7a act as guidance cues/chemoattractants in mammalian neuronal migration. Wnts have previously been implicated in neuronal migrations in Caenorhabditis elegans, where they act as chemorepellents [45, 46]. Although PCP components play a role in FBM migration in zebrafish [1922], no Wnt ligands have so far been linked with the migration (reviewed by [23]). In the mouse, correlative data have showed that in Tbx20 mutant mice with aberrant FBM neuron migration, several Wnt/PCP components are down-regulated [47]. Our study provides functional data suggesting a role for Wnt signalling via a non-canonical pathway, probably involving PCP components, in mammalian FBM migration.

Wnt5a/7a act as guidance cues/chemoattractants

In vertebrates, a role for Wnt5a in cell migration is indicated by the finding that down-regulation of Wnt5a impedes cell migration in a wound-healing assay [48]. Our findings add to a growing body of evidence suggesting that the 'non-canonical Wnts', including Wnt5a, Wnt7a and Wnt11, function in aspects of PCP, including convergent extension movements (for example, [7, 8]), and cochlear hair cell orientation [9, 10]. Intriguingly, Wnt11 is also expressed in FBM neurons, and is absent in Tbx20 mutants [47]. It is possible, therefore, that several Wnts collaborate during the migration. We found that Wnt7a mutant mice had no deficit in FBM migration, whereas in Wnt5a mutants, the migration pattern and nuclear formation were impaired. The relatively weak phenotype in Wnt5a mutants, however, suggests that whereas Wnt5a plays an important role, Wnt7a and/or other Wnts might play an adjunct role. This idea has a parallel in studies demonstrating that Wnt7a and Wnt5a both pattern cochlear hair cell polarisation, but only Wnt5a mutants show cochlear defects [9, 10]. In our system the more uniform expression pattern of Wnt7a might not rule out a possible role in the migration, as in zebrafish, several other Wnt/PCP components that play a functional role in FBM migration are widely expressed in the hindbrain. Interestingly, in the zebrafish, no Wnt ligands have so far been linked to the FBM migration (reviewed by [23]). It remains a formal possibility in the mouse hindbrain that, as has been suggested in other systems, activation of the PCP pathway might depend on a gradient of Fz activity without involving a Wnt ligand [5, 6].

Previous studies on axon guidance in vertebrates have pointed to a role for Wnts in chemoattraction and chemorepulsion of commissural axons and corticospinal axons, respectively, along the rostrocaudal axis of the spinal cord [26, 49]. In both cases Wnts (4 and 1/5a) were proposed to be distributed in a rostralhigh to caudallow gradient, the opposite polarity to that of Wnt5a expression in the hindbrain. It is likely, therefore, that local differences in Wnt gradients along the rostrocaudal axis, coupled with utilisation of different receptors/receptor complexes, dictate different cellular responses. In our proposed model, it is possible that endogenous SFRPs also play a role; SFRP2 is expressed in r4 in the mouse E10.5 hindbrain [50], and might enhance the caudal to rostral Wnt gradient by sequestering Wnt rostrally.

Another reason for the relatively modest phenotype in Wnt5a mutants is likely to be the role played by VEGF in FBM migration. VEGF is expressed in the floor plate and in a lateral stripe in the mouse hindbrain at E12.5, as well as in a group of cells lateral to the FBM nucleus [24]. VEGF acts as a chemoattractant when presented on beads in an explant FBM migration assay [24] and the present study). VEGF and Wnt signalling are therefore likely to co-operate during the process of FBM migration. As Wnt5a is expressed in a rostro-caudal gradient and VEGF is expressed laterally, it may be that Wnts and VEGF play a role in the caudal and the lateral cell displacements, respectively. It is as yet unclear whether they utilise common signalling pathways; our experiments suggest that VEGF does not act via the JNK and ROCK kinases

The PCP pathway is implicated downstream of Wnts

The striking phenotypes of Vangl2 and Scribble mutants, in which FBM migration is arrested, add to a growing body of evidence implicating Wnt/PCP components in FBM migration (Figure 7A, B). The defects we describe are closely similar to those previously shown in the zebrafish Vangl2/Trilobite and Scribble mutants [19, 20, 51]. In zebrafish, in addition to Vangl2/Trilobite and Scribble, mutation of the Wnt/PCP components Prickle, Fz3a and Celsr2 result in FBM migration defects [19, 21, 52]. This raises the likely possibility that mice defective for PCP genes such as Celsr1 [53], which show convergent extension and hair cell polarity defects, might also show aberrant FBM migration.

Figure 7
figure 7

Schematic diagram of facial branchiomotor (FBM) migration and signalling pathways. (A) Schematic diagrams of the final position of the FBM nucleus in embryonic stage (E)14.5 mouse embryos of wild-type, and Wnt5a, Vangl2/Scribble and Fz3 mutant mice. Red patches represent facial nuclei at their final location in wild-type or mutant background. Grey patches show trigeminal nuclei. The red arrow shows the direction of FBM migration in wild-type; the dashed red arrow represents a partial loss of migration in the Wnt 5A-/- mutant; crossed red arrow represents a total loss of migration in the Vangl2/Scrb-/- mutant; the crossed red dashed arrow indicates a loss of migration and partial lateral FBM migration in r5 and r6 in the Fz3-/- mutant. The violet gradient represents the putative distribution of Wnt 5A protein. FP, floor plate. (B) Schematic diagrams of putative Wnt signalling pathways to the cytoskeleton in FBM neurons. Dvl, Dishevelled; Fz, Frizzled; JNK, c-Jun amino-terminal kinase; MLCK, myosin light chain kinase; Pk, Prickle; ROCK, Rho kinase; Scrb, Scribble; Stbm, Strabismus; Vangl2, Van Gogh-like 2.

It appears that at least one Fz protein, Fz3, functions in FBM migration. In Fz3 mutants, as in Vangl2 and Scribble mutants, the majority of FBM neurons migrated laterally (Figure 7A). Fz3 is globally expressed in the mouse hindbrain [54], and in zebrafish, Fz3a has been shown to act non-cell autonomously to control FBM migration [21]. Although we cannot distinguish between a cell autonomous or non-cell autonomous mechanism of action of Fz3 in our system, the two mechanisms may not be mutually exclusive. Fz3 might function in chemoattraction of FBM neurons in a cell-autonomous manner, but a Fz activity gradient in the neuroepithelium might also play a role. This would not exclude the possibility that other Fzs might be involved. Fz4 and Fz7 can interact with Wnt5a, and Fz7 is expressed by FBM neurons [47, 55]. Other studies link the alternative Wnt receptor Ror2 to Wnt5a-induced cell migration [56]. The possibility of an association between Fz and Vangl2 is also suggested by the observation that a multi-PDZ containing protein, MAGI-3, can bind Fz4 and Fz7 and Vangl2, providing the basis of a receptor complex for JNK signalling [57].

JNK and ROCK kinases play a role in FBM migration

Our investigation of downstream signalling in FBM migration by the use of inhibitors revealed a most striking role for JNK, both in the migration per se, and in the deflection of FBM neurons by the presentation of ectopic Wnt5a protein on beads. ROCK inhibition had a less striking effect on the migration pattern, but could also block the Wnt5a effect. Inhibition of MLCK disrupted FBM migration equivalently to ROCK inhibition, but also inhibited the dorsal migration of other hindbrain branchiomotor neurons, suggesting that it does not function specifically in the rostrocaudal migration route. Both JNK and ROCK are well-known to function downstream in the PCP pathway (reviewed by [12, 31]; see Figure 7B for schema). Wnt5a and the PCP pathway have also been linked to both JNK and ROCK in convergent extension movements in fish and frogs [17, 5860]. However, in mammals, PCP downstream signalling pathways have been less clear. Whereas Wnt5a activates JNK in cultured mammalian cells [17, 55], a study focussing on convergent extension in the mouse found a role for RhoA-ROCK, but not JNK, downstream of Vangl2 [15].

We cannot completely rule out the possibility that JNK and ROCK function independently of the PCP pathway in our system. However, on balance, our findings suggest that both JNK and ROCK are important effectors of Wnt5a signalling in FBM neurons. By contrast, MLCK may play a role as a common target for several signalling pathways involved in the migration of branchiomotor (including FBM) neurons. A major target of MLCK is myosin II, which localises to the rear of the nucleus during neuronal migration [61, 62]; mice mutant for myosin IIB show defective facial motor nucleus formation [63, 64], consistent with our MLCK inhibitor studies. The downstream targets of JNK and ROCK phosphorylation in FBM neurons are unknown. Promising candidates are microtubule-associated proteins (MAPs); for example, known targets of JNK are the MAPs MAP1B and doublecortin [65, 66].

Finally, it will be intriguing for future studies to discover whether Wnt5a or other Wnts orchestrate neuronal migrations or axon projection patterns along the rostrocaudal axis of the hindbrain.


In this paper we demonstrate that the Wnt/PCP pathway functions in mammalian FBM migration via an attractant mechanism. Both Wnts are expressed in the hindbrain, and Wnt5a is expressed in a rostrallow to caudalhigh gradient consistent with a chemoattractant role. An in vivo role for Wnts is suggested by the finding that attenuation of endogenous Wnt signalling using SFRPs, or by genetic loss of function of Wnt5a, disrupts the FBM migration. Genetic deletion of the Wnt/PCP genes Fz3, Vangl2 and Scribble leads to severe disruption of FBM migration. We further show that the JNK and ROCK kinases are downstream components of Wnt signalling in FBM neurons. Our study is the first implicating Wnt/PCP signalling in mammalian FBM neuron migration.



embryonic day


facial branchiomotor




c-Jun amino-terminal kinase


microtubule-associated protein


myosin light chain kinase


phosphate-buffered saline


phosphate-buffered saline containing Triton-X100


planar cell polarity




Rho kinase




Semaphorin 3A


secreted frizzed-related protein


Van Gogh-like 2


vascular endothelial growth factor.


  1. Ghashghaei HT, Lai C, Anton ES: Neuronal migration in the adult brain: are we there yet?. Nat Rev Neurosci. 2007, 8: 141-151. 10.1038/nrn2074.

    Article  CAS  PubMed  Google Scholar 

  2. Studer M, Lumsden A, Ariza-McNaughton L, Bradley A, Krumlauf R: Altered segmental identity and abnormal migration of motor neurons in mice lacking Hoxb-1. Nature. 1996, 384: 630-634. 10.1038/384630a0.

    Article  CAS  PubMed  Google Scholar 

  3. McKay IJ, Lewis J, Lumsden A: Organization and development of facial motor neurons in the kreisler mutant mouse. Eur J Neurosci. 1997, 9: 1499-1506. 10.1111/j.1460-9568.1997.tb01504.x.

    Article  CAS  PubMed  Google Scholar 

  4. Auclair F, Valdes N, Marchand R: Rhombomere-specific origin of branchial and visceral motoneurons of the facial nerve in the rat embryo. J Comp Neurol. 1996, 369: 451-461. 10.1002/(SICI)1096-9861(19960603)369:3<451::AID-CNE9>3.0.CO;2-4.

    Article  CAS  PubMed  Google Scholar 

  5. Zallen JA: Planar polarity and tissue morphogenesis. Cell. 2007, 129: 1051-1063. 10.1016/j.cell.2007.05.050.

    Article  CAS  PubMed  Google Scholar 

  6. Jones C, Chen P: Planar cell polarity signaling in vertebrates. Bioessays. 2007, 29: 120-132. 10.1002/bies.20526.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Moon RT, Campbell RM, Christian JL, McGrew LL, Shih J, Fraser S: Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis. Development. 1993, 119: 97-111.

    CAS  PubMed  Google Scholar 

  8. Tada M, Smith JC: Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway. Development. 2000, 127: 2227-2238.

    CAS  PubMed  Google Scholar 

  9. Dabdoub A, Donohue MJ, Brennan A, Wolf V, Montcouquiol M, Sassoon DA, Hseih JC, Rubin JS, Salinas PC, Kelley MW: Wnt signaling mediates reorientation of outer hair cell stereociliary bundles in the mammalian cochlea. Development. 2003, 130: 2375-2384. 10.1242/dev.00448.

    Article  CAS  PubMed  Google Scholar 

  10. Qian D, Jones C, Rzadzinska A, Mark S, Zhang X, Steel KP, Dai X, Chen P: Wnt5a functions in planar cell polarity regulation in mice. Dev Biol. 2007, 306: 121-133. 10.1016/j.ydbio.2007.03.011.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Veeman MT, Axelrod JD, Moon RT: A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling. Dev Cell. 2003, 5: 367-377. 10.1016/S1534-5807(03)00266-1.

    Article  CAS  PubMed  Google Scholar 

  12. Bovolenta P, Rodriguez J, Esteve P: Frizzled/RYK mediated signalling in axon guidance. Development. 2006, 133: 4399-4408. 10.1242/dev.02592.

    Article  CAS  PubMed  Google Scholar 

  13. Torban E, Kor C, Gros P: Van Gogh-like2 (Strabismus) and its role in planar cell polarity and convergent extension in vertebrates. Trends Genet. 2004, 20: 570-577. 10.1016/j.tig.2004.09.003.

    Article  CAS  PubMed  Google Scholar 

  14. Torban E, Wang HJ, Groulx N, Gros P: Independent mutations in mouse Vangl2 that cause neural tube defects in looptail mice impair interaction with members of the Dishevelled family. J Biol Chem. 2004, 279: 52703-52713. 10.1074/jbc.M408675200.

    Article  CAS  PubMed  Google Scholar 

  15. Ybot-Gonzalez P, Savery D, Gerrelli D, Signore M, Mitchell CE, Faux CH, Greene ND, Copp AJ: Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure. Development. 2007, 134: 789-799. 10.1242/dev.000380.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Park M, Moon RT: The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos. Nat Cell Biol. 2002, 4: 20-25. 10.1038/ncb716.

    Article  CAS  PubMed  Google Scholar 

  17. Yamanaka H, Moriguchi T, Masuyama N, Kusakabe M, Hanafusa H, Takada R, Takada S, Nishida E: JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates. EMBO Rep. 2002, 3: 69-75. 10.1093/embo-reports/kvf008.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Higginbotham HR, Gleeson JG: The centrosome in neuronal development. Trends Neurosci. 2007, 30: 276-283. 10.1016/j.tins.2007.04.001.

    Article  CAS  PubMed  Google Scholar 

  19. Bingham S, Higashijima S, Okamoto H, Chandrasekhar A: The zebrafish trilobite gene is essential for tangential migration of branchiomotor neurons. Dev Biol. 2002, 242: 149-160. 10.1006/dbio.2001.0532.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Wada H, Iwasaki M, Sato T, Masai I, Nishiwaki Y, Tanaka H, Sato A, Nojima Y, Okamoto H: Dual roles of zygotic and maternal Scribble1 in neural migration and convergent extension movements in zebrafish embryos. Development. 2005, 132: 2273-2285. 10.1242/dev.01810.

    Article  CAS  PubMed  Google Scholar 

  21. Wada H, Tanaka H, Nakayama S, Iwasaki M, Okamoto H: Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain. Development. 2006, 133: 4749-4759. 10.1242/dev.02665.

    Article  CAS  PubMed  Google Scholar 

  22. Rohrschneider MR, Elsen GE, Prince VE: Zebrafish Hoxb1a regulates multiple downstream genes including prickle1b. Dev Biol. 2007, 309: 358-372. 10.1016/j.ydbio.2007.06.012.

    Article  CAS  PubMed  Google Scholar 

  23. Chandrasekhar A: Turning heads: development of vertebrate branchiomotor neurons. Dev Dyn. 2004, 229: 143-161. 10.1002/dvdy.10444.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Schwarz Q, Gu C, Fujisawa H, Sabelko K, Gertsenstein M, Nagy A, Taniguchi M, Kolodkin AL, Ginty DD, Shima DT, Ruhrberg C: Vascular endothelial growth factor controls neuronal migration and cooperates with Sema3A to pattern distinct compartments of the facial nerve. Genes Dev. 2004, 18: 2822-2834. 10.1101/gad.322904.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Murdoch JN, Henderson DJ, Doudney K, Gaston-Massuet C, Phillips HM, Paternotte C, Arkell R, Stanier P, Copp AJ: Disruption of scribble (Scrb1) causes severe neural tube defects in the circletail mouse. Hum Mol Genet. 2003, 12: 87-98. 10.1093/hmg/ddg014.

    Article  CAS  PubMed  Google Scholar 

  26. Lyuksyutova AI, Lu CC, Milanesio N, King LA, Guo N, Wang Y, Nathans J, Tessier-Lavigne M, Zou Y: Anterior-posterior guidance of commissural axons by Wnt-frizzled signaling. Science. 2003, 302: 1984-1988. 10.1126/science.1089610.

    Article  CAS  PubMed  Google Scholar 

  27. Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L: Wnt3a binds to several sFRPs in the nanomolar range. Biochem Biophys Res Commun. 2007, 357: 1119-1123. 10.1016/j.bbrc.2007.04.069.

    Article  CAS  PubMed  Google Scholar 

  28. Shamim H, Mahmood R, Mason I: In situ hybridization to RNA in whole embryos. Methods Mol Biol. 1999, 97: 623-633.

    CAS  PubMed  Google Scholar 

  29. Myat A, Henrique D, Ish-Horowicz D, Lewis J: A chick homologue of Serrate and its relationship with Notch and Delta homologues during central neurogenesis. Dev Biol. 1996, 174: 233-247. 10.1006/dbio.1996.0069.

    Article  CAS  PubMed  Google Scholar 

  30. Varela-Echavarria A, Pfaff SL, Guthrie S: Differential expression of LIM homeobox genes among motor neuron subpopulations in the developing chick brain stem. Mol Cell Neurosci. 1996, 8: 242-257. 10.1006/mcne.1996.0061.

    Article  CAS  PubMed  Google Scholar 

  31. Ciani L, Salinas PC: WNTs in the vertebrate nervous system: from patterning to neuronal connectivity. Nat Rev Neurosci. 2005, 6: 351-362. 10.1038/nrn1665.

    Article  CAS  PubMed  Google Scholar 

  32. Studer M: Initiation of facial motoneurone migration is dependent on rhombomeres 5 and 6. Development. 2001, 128: 3707-3716.

    CAS  PubMed  Google Scholar 

  33. Kibar Z, Vogan KJ, Groulx N, Justice MJ, Underhill DA, Gros P: Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail. Nat Genet. 2001, 28: 251-255. 10.1038/90081.

    Article  CAS  PubMed  Google Scholar 

  34. Gilardi-Hebenstreit P, Nieto MA, Frain M, Mattei MG, Chestier A, Wilkinson DG, Charnay P: An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain. Oncogene. 1993, 8: 1103-

    CAS  PubMed  Google Scholar 

  35. Wilkinson DG, Bhatt S, Chavrier P, Bravo R, Charnay P: Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse. Nature. 1989, 337: 461-464. 10.1038/337461a0.

    Article  CAS  PubMed  Google Scholar 

  36. Hammond R, Vivancos V, Naeem A, Chilton J, Mambetisaeva E, Andrews W, Sundaresan V, Guthrie S: Slit-mediated repulsion is a key regulator of motor axon pathfinding in the hindbrain. Development. 2005, 132: 4483-4495. 10.1242/dev.02038.

    Article  CAS  PubMed  Google Scholar 

  37. Simon H, Guthrie S, Lumsden A: Regulation of SC1/DM-GRASP during the migration of motor neurons in the chick embryo brain stem. J Neurobiol. 1994, 25: 1129-1143. 10.1002/neu.480250908.

    Article  CAS  PubMed  Google Scholar 

  38. Goddard JM, Rossel M, Manley NR, Capecchi MR: Mice with targeted disruption of Hoxb-1 fail to form the motor nucleus of the VIIth nerve. Development. 1996, 122: 3217-3228.

    CAS  PubMed  Google Scholar 

  39. Varela-Echavarria A, Tucker A, Puschel AW, Guthrie S: Motor axon subpopulations respond differentially to the chemorepellents netrin-1 and semaphorin D. Neuron. 1997, 18: 193-207. 10.1016/S0896-6273(00)80261-5.

    Article  CAS  PubMed  Google Scholar 

  40. Kawano Y, Kypta R: Secreted antagonists of the Wnt signalling pathway. J Cell Sci. 2003, 116: 2627-2634. 10.1242/jcs.00623.

    Article  CAS  PubMed  Google Scholar 

  41. Bovolenta P, Esteve P, Ruiz JM, Cisneros E, Lopez-Rios J: Beyond Wnt inhibition: new functions of secreted Frizzled-related proteins in development and disease. J Cell Sci. 2008, 121: 737-746. 10.1242/jcs.026096.

    Article  CAS  PubMed  Google Scholar 

  42. Montcouquiol M, Sans N, Huss D, Kach J, Dickman JD, Forge A, Rachel RA, Copeland NG, Jenkins NA, Bogani D, Murdoch J, Warchol ME, Wenthold RJ, Kelley MW: Asymmetric localization of Vangl2 and Fz3 indicate novel mechanisms for planar cell polarity in mammals. J Neurosci. 2006, 26: 5265-5275. 10.1523/JNEUROSCI.4680-05.2006.

    Article  CAS  PubMed  Google Scholar 

  43. Wang Y, Guo N, Nathans J: The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells. J Neurosci. 2006, 26: 2147-2156. 10.1523/JNEUROSCI.4698-05.2005.

    Article  CAS  PubMed  Google Scholar 

  44. Wang Y, Thekdi N, Smallwood PM, Macke JP, Nathans J: Frizzled-3 is required for the development of major fiber tracts in the rostral CNS. J Neurosci. 2002, 22: 8563-8573.

    CAS  PubMed  Google Scholar 

  45. Pan CL, Howell JE, Clark SG, Hilliard M, Cordes S, Bargmann CI, Garriga G: Multiple Wnts and frizzled receptors regulate anteriorly directed cell and growth cone migrations in Caenorhabditis elegans. Dev Cell. 2006, 10: 367-377. 10.1016/j.devcel.2006.02.010.

    Article  CAS  PubMed  Google Scholar 

  46. Zou Y: Navigating the anterior-posterior axis with Wnts. Neuron. 2006, 49: 787-789. 10.1016/j.neuron.2006.03.004.

    Article  CAS  PubMed  Google Scholar 

  47. Song MR, Shirasaki R, Cai CL, Ruiz EC, Evans SM, Lee SK, Pfaff SL: T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. Development. 2006, 133: 4945-4955. 10.1242/dev.02694.

    Article  CAS  PubMed  Google Scholar 

  48. Cheng CW, Yeh JC, Fan TP, Smith SK, Charnock-Jones DS: Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration. Biochem Biophys Res Commun. 2008, 365: 285-290. 10.1016/j.bbrc.2007.10.166.

    Article  CAS  PubMed  Google Scholar 

  49. Liu Y, Shi J, Lu CC, Wang ZB, Lyuksyutova AI, Song XJ, Zou Y: Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract. Nat Neurosci. 2005, 8: 1151-1159. 10.1038/nn1520.

    Article  CAS  PubMed  Google Scholar 

  50. Leimeister C, Bach A, Gessler M: Developmental expression patterns of mouse sFRP genes encoding members of the secreted frizzled related protein family. Mech Dev. 1998, 75: 29-42. 10.1016/S0925-4773(98)00072-0.

    Article  CAS  PubMed  Google Scholar 

  51. Jessen JR, Topczewski J, Bingham S, Sepich DS, Marlow F, Chandrasekhar A, Solnica-Krezel L: Zebrafish trilobite identifies new roles for Strabismus in gastrulation and neuronal movements. Nat Cell Biol. 2002, 4: 610-615.

    PubMed Central  CAS  PubMed  Google Scholar 

  52. Carreira-Barbosa F, Concha ML, Takeuchi M, Ueno N, Wilson SW, Tada M: Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish. Development. 2003, 130: 4037-4046. 10.1242/dev.00567.

    Article  CAS  PubMed  Google Scholar 

  53. Curtin JA, Quint E, Tsipouri V, Arkell RM, Cattanach B, Copp AJ, Henderson DJ, Spurr N, Stanier P, Fisher EM, Nolan PM, Steel KP, Brown SD, Gray IC, Murdoch JN: Mutation of Celsr1 disrupts planar polarity of inner ear hair cells and causes severe neural tube defects in the mouse. Curr Biol. 2003, 13: 1129-1133. 10.1016/S0960-9822(03)00374-9.

    Article  CAS  PubMed  Google Scholar 

  54. Tissir F, Goffinet AM: Expression of planar cell polarity genes during development of the mouse CNS. Eur J Neurosci. 2006, 23: 597-607. 10.1111/j.1460-9568.2006.04596.x.

    Article  PubMed  Google Scholar 

  55. Oishi I, Suzuki H, Onishi N, Takada R, Kani S, Ohkawara B, Koshida I, Suzuki K, Yamada G, Schwabe GC, Mundlos S, Shibuya H, Takada S, Minami Y: The receptor tyrosine kinase Ror2 is involved in non-canonical Wnt5a/JNK signalling pathway. Genes Cells. 2003, 8: 645-654. 10.1046/j.1365-2443.2003.00662.x.

    Article  CAS  PubMed  Google Scholar 

  56. Nishita M, Yoo SK, Nomachi A, Kani S, Sougawa N, Ohta Y, Takada S, Kikuchi A, Minami Y: Filopodia formation mediated by receptor tyrosine kinase Ror2 is required for Wnt5a-induced cell migration. J Cell Biol. 2006, 175: 555-562. 10.1083/jcb.200607127.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  57. Yao R, Natsume Y, Noda T: MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap. Oncogene. 2004, 23: 6023-6030. 10.1038/sj.onc.1207817.

    Article  CAS  PubMed  Google Scholar 

  58. Kim GH, Han JK: JNK and ROKalpha function in the noncanonical Wnt/RhoA signaling pathway to regulate Xenopus convergent extension movements. Dev Dyn. 2005, 232: 958-968. 10.1002/dvdy.20262.

    Article  CAS  PubMed  Google Scholar 

  59. Zhu S, Liu L, Korzh V, Gong Z, Low BC: RhoA acts downstream of Wnt5 and Wnt11 to regulate convergence and extension movements by involving effectors Rho kinase and Diaphanous: use of zebrafish as an in vivo model for GTPase signaling. Cell Signal. 2006, 18: 359-372. 10.1016/j.cellsig.2005.05.019.

    Article  CAS  PubMed  Google Scholar 

  60. Schambony A, Wedlich D: Wnt-5A/Ror2 regulate expression of XPAPC through an alternative noncanonical signaling pathway. Dev Cell. 2007, 12: 779-792. 10.1016/j.devcel.2007.02.016.

    Article  CAS  PubMed  Google Scholar 

  61. Schaar BT, Kinoshita K, McConnell SK: Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons. Neuron. 2004, 41: 203-213. 10.1016/S0896-6273(03)00843-2.

    Article  CAS  PubMed  Google Scholar 

  62. Bellion A, Baudoin JP, Alvarez C, Bornens M, Metin C: Nucleokinesis in tangentially migrating neurons comprises two alternating phases: forward migration of the Golgi/centrosome associated with centrosome splitting and myosin contraction at the rear. J Neurosci. 2005, 25: 5691-5699. 10.1523/JNEUROSCI.1030-05.2005.

    Article  CAS  PubMed  Google Scholar 

  63. Ma X, Kawamoto S, Hara Y, Adelstein RS: A point mutation in the motor domain of nonmuscle myosin II-B impairs migration of distinct groups of neurons. Mol Biol Cell. 2004, 15: 2568-2579. 10.1091/mbc.E03-11-0836.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  64. Ma X, Kawamoto S, Uribe J, Adelstein RS: Function of the neuron-specific alternatively spliced isoforms of nonmuscle myosin II-B during mouse brain development. Mol Biol Cell. 2006, 17: 2138-2149. 10.1091/mbc.E05-10-0997.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  65. Chang L, Jones Y, Ellisman MH, Goldstein LS, Karin M: JNK1 is required for maintenance of neuronal microtubules and controls phosphorylation of microtubule-associated proteins. Dev Cell. 2003, 4: 521-533. 10.1016/S1534-5807(03)00094-7.

    Article  CAS  PubMed  Google Scholar 

  66. Gdalyahu A, Ghosh I, Levy T, Sapir T, Sapoznik S, Fishler Y, Azoulai D, Reiner O: DCX, a new mediator of the JNK pathway. EMBO J. 2004, 23: 823-832. 10.1038/sj.emboj.7600079.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references


We are grateful to Dr Dawn Savery and Dr Andrew Copp for initial help with obtaining Vangl2 and Scribble mutant mice. We thank Dr Lorenza Ciani and Dr Patricia Salinas for providing reagents, Wnt7a mutant mice and helpful advice. Thanks also to Dr Britta Eickholt for help with inhibitor studies, and to Dr Uwe Drescher for valuable discussions on the manuscript. We thank the Wellcome Trust very much for funding the work described in this article.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Sarah Guthrie.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

VV performed all the experiments reported in this paper and developed the concepts. SG is the senior author, in whose laboratory the work was done, and who conceived the project together with MS, a co-grant holder for the project. PC, DQ, AD and MK provided Wnt5a mutant mice, while NS provided Frizzled3 mutant mice.

Electronic supplementary material


Additional file 1: Quantification of FBM migration in explants containing Wnt- or PBS-treated beads. (A) Example of FBM neurons labelled using anti-Islet-1/2 antibody in control explant cultured for 48 hours. Position of beads shown by white circles. Area quantified by pixel counting is shown by the red box and encompasses the beads themselves located at the r3/r4 boundary, and the ipsilateral r4 up to the midline. (B) Quantification of pixels adjacent to Wnt5a or control beads, or Wnt5a beads in the presence of JNK inhibitor SP600125 or ROCK inhibitor Y27632. N = 5 explants in each case. Statistical comparison of control versus Wnt5a p < 0.01 (indicated by asterisk); Wnt5a versus SP600125 p < 0.01 (indicated by asterisk); and Wnt5a versus Y27632, p < 0.05 (indicated by asterisk). (C) Scoring of explants containing VEGF-treated beads and VEGF-treated beads with Y27632 inhibitor. N = 6–10 explants; p > 0.05. (D) Scoring of explants containing VEGF-treated beads and VEGF-treated beads with SP600125. N = 6–10 explants; p > 0.05. (E) Example of control explant cultured for 48 hours showing boxes (outlined in red) in r4 and r6 used for pixel counting to quantify migration. Ratio of r6 pixel counts/r4 pixel counts was derived for each explant. (F) Quantification of the mean ratio of pixels r6/r4 for control explants and explants treated with SP600125 and Y27632 inhibitors. Control versus SP600125 p < 0.01 (indicated by asterisk); control versus Y27632 p < 0.05 (indicated by asterisk). Scale bars: 250 μm in (A); 300 μm in (E). (PDF 125 KB)


Additional file 2: Expression pattern of genes in Vangl2 and Scribble mutants. (A, B) Flat-mounted hindbrains of E11.5 mouse embryos in situ-hybridised for EphA4 in wild type (A) and its Scrb-/- homozygous mutant litter-mate (B), showing that rhombomere segmentation is conserved in the mutant background. (C-F) Transverse cryosections of E11.5 wild type and Vangl2-/- homozygous mutants at the r4 level, in situ hybridised for Wnt5A (C, D) and Slit1 probes (E, F), showing that no difference in expression pattern was observed. Scale bars: 250 μm (A, B); 500 μm (C-F). (PDF 232 KB)


Additional file 3: Quantification of the dorsal migration of trigeminal branchiomotor neurons in explants treated with inhibitors. The dorsal migration of trigeminal motor neurons was scored on a 1–3 scale as for the FBM migration. Axes as in Figure 6. (A) JNK inhibitor SP600125, (B) ROCK inhibitor Y27632, (C) MLCK inhibitor ML-7. Inhibitor-treated versus control p > 0.05 (A, B), p < 0.001 (indicated by asterisk) (C). N = 20 explants in each group. (PDF 12 KB)

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Vivancos, V., Chen, P., Spassky, N. et al. Wnt activity guides facial branchiomotor neuron migration, and involves the PCP pathway and JNK and ROCK kinases. Neural Dev 4, 7 (2009).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: