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Figure 2 | Neural Development

Figure 2

From: Wnt activity guides facial branchiomotor neuron migration, and involves the PCP pathway and JNK and ROCK kinases

Figure 2

Chemoattractant effect of Wnt proteins in hindbrain explants. (A-B') Embryonic stage (E)11.5 mouse hindbrain explants with Wnt-coated beads placed in a region of rhombomere (r)4 rostral and lateral to the facial branchiomotor migratory stream: (A) phosphate-buffered saline (PBS) control; (B) Wnt protein; (A', B') higher magnifications of regions in boxes in (A, B), respectively. White circles mark beads. Scale bars: 250 μm in (A, B); 125 μm in (A', B'). (C, D) Quantification of bead experiments, with proteins as indicated. (C) Wnt7a versus control p < 0.001, Wnt5a versus control p < 0.001 (indicated by asterisks), n = 25 explants in each group. (D) Vascular endothelial growth factor (VEGF) versus control p < 0.01 (positive control; indicated by single asterisk). Semaphorin 3A (Sema3A) versus control p > 0.05 (negative control; indicated by double asterisks), n = 10 explants in each group.

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Neural Development

ISSN: 1749-8104