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Figure 4 | Neural Development

Figure 4

From: Wnt activity guides facial branchiomotor neuron migration, and involves the PCP pathway and JNK and ROCK kinases

Figure 4

Analysis of Vangl2 and Scribble mutant phenotypes. (A, B, D, E, G, H, I-K) In situ hybridization with Islet-1 probe on flat-mount hindbrains showing facial branchiomotor (FBM) migration in wild-type embryos, and Vangl2 and Scribble heterozygotes and homozygotes. Embryonic stages (E) and probes are as indicated. Both homozygote mutants present a very dramatic failure of FBM migration (asterisks in (D, E, G, H)) with a population of motor neurons in the floor plate (FP, arrowheads). (J, K) In heterozygous Vangl2 and Scribble (Scrb) littermates at E13.5, a stream of cells arrest in the dorsal region of rhombomere (r)5 (arrowheads) compared to wild type (WT) (C, F) Transverse sections through the hindbrains of wild-type (C) and Vangl2-/- (F) hindbrains, immunostained with anti-Islet1/2 antibody to show FBM neurons migrating laterally in r6 (C) to form the FBM nucleus (asterisk) or remaining in r4 (asterisk in F). ( I). Scale bars: 250 μm in (A, B, D, E, G, H); 100 μm in (C, F); 125 μm in (I-K). TN, trigeminal motor nucleus.

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