Figure 6

Inhibition of facial branchiomotor (FBM) migration and of Wnt-mediated attraction. (A, D, G) Control explants cultured for 48 hours and immunostained with anti-Islet 1/2 antibody with FBM neurons located in their final position forming the facial motor nuclei in rhombomere (r)6. (B, E, H) Explants treated with c-Jun amino-terminal kinase (JNK) inhibitor (SP600125), Rho kinase (ROCK) inhibitor (Y27632) and myosin light chain kinase inhibitor (ML-7) as labelled. Scale bars: 250 μm (A, B, D, E, G, H). (C, F, I) Quantification of the effects of inhibitors on FBM migration in explants. The x-axis is the migration scale where 1 = loss of migration, 2 = intermediate loss of migration, 3 = normal migration. The y-axis is the percentage of explants in a particular category. Inhibitor-treated versus control p < 0.001 (indicated by asterisk). (J, K) Quantification of the effect of inhibitors on Wnt5A attractive beads. The attractive effect of Wnt5A ectopic beads is inhibited by adding inhibitors SP600125 (J) and Y27632 (K). Inhibitor-treated versus control p > 0.05 (indicated by asterisk). (C, F, I, J, K) N = 15–20 explants in each group.