Fig. 3

Cell proliferation state in neuroprogenitors in Ntn1, Dcc, and Robo3 knockouts. a Cross sections of the spinal cord from embryos cultured for 20 h. The closeup images are of the boxed area and the dashed line outlines the VZ. Anti-Ki67 staining marks neurons that are inside the cell cycle. b Quantification of the ratio between GFP+ Ki67+ neurons and the total GFP+ neurons within the VZ. The percentage of neurons that are inside the cell cycle is comparable between all three KOs and WT controls. Data are represented as the mean ± SEM (Student’s t-test; ns, not significant). Scale bars, 50 μm