Fig. 4

Three functional lmx1b alleles are required for correct numbers of glutamatergic cells at later developmental stages. Lateral view of zebrafish spinal cord at 48 h (a-g’, i and j), anterior left, dorsal top. in situ hybridization for slc17a6a + slc17a6b (slc17a6) (a-g’) and slc32a1 (i and j). (a’-g’) are magnified views of black dashed box regions in panels (a-g). Columns on left indicate lmx1ba and lmx1bb genotype. Mean number of cells (y-axis) expressing slc17a6 (h) and slc32a1 (k) in spinal cord region adjacent to somites 6–10 at 48 h (x-axis). Square brackets and star in (h) indicates that each of the first three columns is statistically significantly different from each of the last four columns (p < 0.05). Embryo genotype is indicated below graph. Error bars indicate standard error of the mean. Two independent experiments were conducted for (a-g). Cell count results were similar in each replicate. One experiment was conducted for (i and j). Cell count data presented here (h and k) are average values of 4–13 embryos. Precise numbers of embryos counted and p values are provided in Table 5. Scale bar (g) = 50 μm (a-g) and 20 μm (a’-g’) and scale bar (j) = 50 μm (i, j)