Figure 4From: The chromatin remodeling factor Bap55 functions through the TIP60 complex to regulate olfactory projection neuron dendrite targetingBap55 acts postmitotically in PNs and dendrite mistargeting can be suppressed by human BAF53a and b. (A, B) Postmitotic expression of UAS-Bap55 can rescue the Bap55-/-dendrite mistargeting phenotype. The PN no longer targets to the anterior glomerulus DA4l (A1), but to the posterior glomerulus DL1 (A2). The L-shaped axon pattern is not altered (B). (C, D) In a small number of cases, postmitotic expression of UAS-Bap55 in a Bap55-/-single cell causes a de novo phenotype. The dendrites target to the anterior and medial glomerulus DM6 (C1), abandoning both DA4l (C1) and DL1 (C2). In these cases, it also causes a de novo axon phenotype of mistargeting ventrally to the lateral horn (white arrow in (D)). (E, F) Postmitotic expression of human UAS-Baf53a also rescues the Bap55-/-phenotype (E) and does not alter the L-shaped axon pattern (F). (G, H) Postmitotic expression of human UAS-Baf53b also rescues the Bap55-/-phenotype (G) and does not alter the L-shaped axon pattern (H). (I) Quantification of mistargeting phenotypes for (A, C, E, G). Green marks mCD8-GFP-labeled PNs generated by MARCM and labeled using GH146-GAL4. (A, C, E, G) show partial confocal stacks; (B, D, F, H) show full confocal stacks; magenta is the presynaptic marker nc82; symbols are as in Figure 2. Scale bars: 20 μm in (A) (for A, C, E, G) and (B) (for B, D, F, H).Back to article page