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Figure 3 | Neural Development

Figure 3

From: Slit and Netrin-1 guide cranial motor axon pathfinding via Rho-kinase, myosin light chain kinase and myosin II

Figure 3

Cranial motor axon repulsion is blocked by anti-Unc5a antibodies and/or anti-Robo1/2 antibodies in a floor plate deflection assay. (A) Hindbrain control (Cont) explant containing the floor plate (FP) and BM neurons immunostained with anti-SC1 antibodies. (B) Hindbrain explant cultured with juxtaposed floor plate shows motor axonal deflection θ. (C) Application of anti-Unc5a antibodies reduces this deflection. (D) Application of anti-Unc5a + anti-Robo1/2 antibodies together reduces the deflection further. Dotted lines show floor plate-explant border. Scale bar = 100 μm. (E) Graph showing the mean angle of deflection in the presence of various antibodies (N = 40 to 50 explants in each category). Asterisks denote categories showing blockade of repulsion, that is, reduction of axon deflection to level of 'Control no FP'.

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Neural Development

ISSN: 1749-8104