Figure 4

Genes enriched in the odd-numbered rhombomeres (r3 and r5). (A-G) The normalised expression is represented graphically for each rhombomere +/- SEM. (A'-G') Hindbrain flat-mount preparations of the corresponding gene. (A'-A"') The r3/5 patterning gene has a dynamic expression pattern in the hindbrain between E8.5 and E9.5 (blue arrows). At the time of the tissue harvesting (E9.5), Egr2 was down-regulated in r3 and restricted to dorsal r5 territories (A"', black arrows). Genes potentially enriched in odd-numbered rhombomeres were selected for verification based upon their molecular functions and predicted expression profiles. The expression profiles are consistent with the derived microarray output.