Figure 5

Cell cycle exit was unchanged in the EIIa-Cre;Fgfr3^+/K644Ecortical primordium at E12.5. (a, b) Double immunohistochemistry labeled bromodeoxyuridine⁺ (BrdU⁺; green) and Ki67⁺ (red) cells 24 hours after BrdU injection. Cells labeled with BrdU can either remain in the cell cycle (BrdU⁺Ki67⁺; yellow, arrowheads) or exit the cell cycle (BrdU⁺Ki67^-; green, asterisks). Panels (a, b) are representative images of the dorsal region of the caudal cortical primordium in wild-type (Wt) and EIIa-Cre;Fgfr3^+/K644E, respectively. (c) Cells that had exited the cell cycle were counted within a 50 μm channel in the dorsal region and their percentage was calculated by dividing the number of BrdU⁺Ki67^- cells by that of BrdU⁺ cells (n = 6; p = 0.022, Student's t- test, indicated by *). Three embryos per genotype and two counting boxes per sample were used (n = 6). Scale bar: 35 μm.