Figure 4
From: Fgf receptor 3 activation promotes selective growth and expansion of occipitotemporal cortex
Reduced cell cycle length in the caudal region of the EIIa-Cre;Fgfr3+/K644Ecortical primordium at E12.5. (a, b) Cumulative bromodeoxyuridine (BrdU) labeling was performed at 8 time points between 0.5 and 11 hours at E12.5. Four embryos per genotype and two counting boxes per sample were used at each time point. Panels (a, b) are representative wild-type coronal forebrain sections at the rostral (a) and caudal levels (b). Equivalent levels of sections in EIIa-Cre;Fgfr3+/K644Ewere selected using morphological landmarks. Scale bar: 670 μm. (c, d) LI of EIIa-Cre;Fgfr3+/K644E(black circles) and wild type (Wt; open squares) was plotted against each time point at the rostral (c) and caudal levels (d). In contrast to the rostral region, which showed similar profiles in EIIa-Cre;Fgfr3+/K644Eand wild type (c), the LI plateau was reached at an earlier time point in EIIa-Cre;Fgfr3+/K644Ecompared to wild type in the caudal region (d), indicating a shorter Tc-Ts.