Figure 3
From: Fgf receptor 3 activation promotes selective growth and expansion of occipitotemporal cortex
Little change in gene expression patterning in the EIIa-Cre;Fgfr3+/K644Ecortical primordium. (a-d) Expression of Fgf8 was examined by whole mount in situ hybridization at E9.5. Focal expression of Fgf8 in the anterior neural ridge was observed as expected (white arrowhead) in wild type (Wt) (a, c), and a similar expression domain and level of Fgf8 was observed in EIIa-Cre;Fgfr3+/K644E(b, d). (e, f, m-p) Coup-Tf1 was expressed similarly in wild type (e, m, o) and EIIa-Cre;Fgfr3+/K644E(f, n, p) at E11.5 (g, h) and E13.5 (m-p). Panels (o, p) are medial views of (m, n), respectively. (g, h) Wnt2b marked the caudal limit of the cortical hem (arrows), indicating a selective expansion of the caudal region in EIIa-Cre;Fgfr3+/K644E. Note the overall reduction in expression level of Wnt2b in (h). No obvious difference was observed in expression of Pax6 at E11.5 (i, j) and Emx2 at E11.5 (k, l) between the wild-type (i, k) and EIIa-Cre;Fgfr3+/K644Esamples (j, l). Results presented here are representatives of at least three independent hybridizations (n > 3). Scale bars: 250 μm in (a, b); 100 μm in (c, d); 400 μm in (e, f, i-l); 800 μm in (g, h, m-p).