Figure 5

Dendritic arborization (da) neurons in the adult. (A) A lateral image of a whole-mount abdomen of a 1- to 3-day-old female of genotype UAS-RedStinger/+; Gal4^109(2)80UAS-mCD8::GFP/+. RedStinger encodes a nuclear targeted DsRed. The yellow rectangle indicates A4. oe, oenocyte; sp, spiracle. (B) A ventral-lateral image of the pleura of a 3- to 4-day-old male of ppk-mCD8::3xEGFP/ppk-mCD8::3xEGFP. The pleura was covered with lattice-shaped dendritic arbors of v'ada neurons (yellow arrows), and a couple of branches in each arbor were more strongly labeled than the others. (C-J) High-power images of the tergite (C-E) and those of the pleura and the sternite (G-I) in A3/A4 of another whole-mount female of the same genotype as in (A). The channel signals of GFP (C, G) (green in (E, I)) and DsRed (D, H) (magenta or white in (E, I)) are shown. Yellow arrows point to nuclei of da neurons, and blue ones to those of dorsal bipolar dendrite (dbd) neurons. Nuclei of ldaA and ldaA-like are closely associated with each other (G-I; the inset in (I) shows a higher-power image of a different hemisegment). (F, J) Tracings of dendrites and cell bodies in (C) and (G), respectively. (K-M) A fillet preparation of a pharate adult female of genotype Gal4^109(2)80UAS-mCD8::GFP/Gal4^109(2)80UAS-mCD8::GFP. mCD8 staining (K) (green in (M)) and Elav staining (L) (white in (M)), and a merged image (M). This high-power image highlights a pair of nuclei of ldaA and ldaA-like. Scale bars: 50 μm (c-m).