Figure 2

Distribution of peripheral neurons in the abdomen of the pharate adult. (A) A lateral view of a pharate adult that was taken out of its puparium. Broken lines show how we dissected it. (B) A bright-field image of a fillet preparation of the abdomen of a male. Orange broken lines indicate boundaries between tergite, pleura, and sternite; green broken lines here and in (D) indicate dorsal and ventral midlines. The boxed region is highlighted in Figure 3. (C) Dark belts in the adult abdomen indicate oenocytes (oe). Adapted from FlyBase. sp, spiracle. (D-F) Double labeling of a fillet preparation of a male with anti-mCD8 antibody (D) (green in (F)) and a pan-neuronal marker, anti-HRP antibody (E) (magenta or white in (F)), of genotype Gal4^109(2)80UAS-mCD8::GFP/Gal4^109(2)80UAS-mCD8::GFP. dv, dorsal vessel (heart). The boxed region in (E) is highlighted in Additional file 4AA. In (F), A2 to A6 indicate numbers of abdominal segments. Yellow arrows point to identified dendritic arborization (da) neurons. Blue arrows indicate dorsal bd neurons (dbds), and blue arrowheads bipolar neurons that innervate the abdominal heart (BpN) [34]. Yellow arrowheads point to representative external sensory (es) neurons, and double yellow arrowheads a pair of v'esB located on both sides of the ventral midline [25]. Asterisks indicate examples of mCD8::GFP-positive cells that were associated with fascicles. Scale bars: 50 μm.