Figure 7

Broadening of the Islet-1-expressing corridor in E12.5 conditional knockouts. (A) Coronal section of the telencephalon of an E12.5 control embryo showing immunohistochemistry for Islet1; the arrangement of bins used for counting Islet1-expressing cells across the corridor in control and conditional knockout (cKO) embryos is marked. (B) Graph shows the distributions of Islet1-expressing cells across the corridor: values plotted are mean densities per bin ± standard error of the mean (n = 4 embryos for both control and cKO data); black error bars and grey shading are for control data; red error bars and broken line without shading are for cKO data; double-headed arrows indicate the widths of the distributions at half their peak values (black arrow for control data, red arrow for cKO data). (C, D) Illustration of the finding: in cKO embryos Islet1-expressing cells spread more broadly from the corridor towards the ventral surface of the brain (at the bottom left of each panel) than in controls. Scale bars: 50 μm.