Re-modeling of the CSD neuron during pupal life. (a-d) Developmental changes in different regions of the CSD neuron; (a) cell body and dendrites within the ipsilateral lobe, (b) presynaptic terminals in the contralateral lobe, (c) branches in the ipsilateral higher centers, and (d) branches in the contralateral higher centers. The regions are shown at (i) 0 hours APF, (ii) 10 hours APF, (iii) 40 hours APF, (iv) 80 hours APF and (v) adult. Scale bar = 5 μm in (i) and (ii); 10 μm in (iii); 40 μm in (iv) and (v). Yellow arrows indicate branches being pruned while green arrows point to the new sprouts. The branching has been quantified as described in Additional file 3 and plotted in histograms in (a(vi)), (b(vi)), (c(vi)) and (d(vi)). The number of branch points is represented at different time points; bars indicate the mean and standard error of mean of five preparations in each case. (e) Infiltration of glial cells at the sites of pruning of the CSD neuron at (i) 10 hours and (ii) 20 hours APF. The glial cells are visualized by staining with an antibody against Repo (red). The means and standard error of mean of the number of cells in five preparations are represented in the histogram in (e(iv)).