Fig. 3

MAP2, Hu C/D and neurofilament expression in the preplate and cortical plate of the developing GCR neocortex. A–H Combined immunofluorescence for NF and HUCD (red), and MAP2 (green) and DAPI staining (blue) on 30 µm cryosections of GD60-140 GCR neocortex. A Images show neurons with Hu C/D + soma (open arrowhead) in higher magnification of GD60 GCR neocortex. Scale bar, 100 µm. B–D The bottom margin corresponds to the apical boundary of the preplate (B) or the transition zone SP/CP (C, D). Scale bars, 50 µm. E Images show neurons with Hu C/D + soma (open arrowhead) and extending Map2 + dendrites (solid arrowhead) in higher magnification of GD130 GCR neocortex. Scale bar, 100 µm. F–H The bottom margin corresponds to the transition zone SP/CP. Scale bars, 100 µm. A–H PP, preplate; CP, cortical plate; SP, subplate