Fig. 1
From: Neocortex neurogenesis and maturation in the African greater cane rat
Pax6 and Tbr2 expression in the germinal zones of the developing GCR neocortex. A–I Double-Immunofluorescence for Pax6 (red) and Tbr2 (green) and DAPI staining (blue) on 20 µm cryosections of GD50 and 30 µm cryosections of GD60-140 GCR neocortex. Scale bars, 25 µm. A, B The entire cortical is shown. C–I The top margin of the image corresponds to the transition zone SVZ/intermediate zone. J Quantification of the radial thickness of the germinal zones in the developing GCR neocortex. K, L Quantification of Pax6 + /Tbr2– (red), Pax6 + /Tbr2 + (brown) and Pax6–/Tbr2 + (green) NPCs in the VZ (K) and SVZ (L), expressed as number of cells per 100 µm ventricular surface. Cortical wall corresponding to a total ventricular surface of 309–1426 µm was analyzed. (J–L) Data represent mean ± SEM and are from two fetuses each. VZ, ventricular zone; SVZ/IZ, subventricular zone/intermediate zone; CP, cortical plate