Fig. 4

Analysis of ∆18 heterozygous neurons shows the bushy phenotype is neither strictly recessive nor dominant. Data from two independent experiments (a-d and e–h, respectively), each with parallel 3-div cultures of larval CNS neurons from CASK control (Ex33/Ex33; aqua), CASK mutant (Δ18/∆18; magenta), and heterozygote (Δ18/Ex33; burgundy). Box-plot distributions depicted as in Fig. 3, with n = 101–104 neurons from each genotype in each experiment. a, e Total neurite length; b, f territory area; c, g higher-order branch number; and d, h branch density. For two key characteristic features of the bushy phenotype, decreased territory area and increased branch density, ∆18 was dominant (b, d) or semi-dominant (f, h). Decreased total neurite length showed opposite results in the two experiments (a, e) and decreased higher-order branch number appeared to be recessive (c, g). Significance levels: *, p < 0.05; ***, p < 0.0005; ****, p < 0.00005