Fig. 8

Tcf4 is expressed in Tcf3/12 dcKOs. At E15.5, Tcf4 is expressed within the MGE in controls (A). This expression is also seen in the dcKOs, although more Tcf4 expression is seen due to increased MGE size (B). However, when controlling for size of the MGE, there is no difference in Tcf4 expression (Quantification for total intensity and intensity normalized to area - C). At P21, less Tcf4+ cells are detected in the corpus callosum in dcKOs compared to controls (D-E). Since there are overall fewer OLs in the corpus callosum, Tcf4 expression was normalized to percentage of Olig2+ cells co-expressing Tcf4. Once normalized, Tcf4 expression had no significant difference between controls and dcKOs (Quantification for average cell numbers and normalized percentage – F). Tcf4 is highly expressed in the cortex under normal conditions, and no difference is observed between controls and dcKOs (G-H, Quantification – I).  Scale bars B = 200 μm (for A-B), E = 100 μm (for D-E), and H= 100 μm (for G-H)