Fig. 5

Reduction of mature oligodendrocytes in Tcf3/12 dcKOs persists at later postnatal stages. At P21, reduction of Plp1 expression in dcKOs compared to controls is still observed (A-B,O). At this stage, protein expression for myelin markers, CNPase and MBP are also reduced in the cortex of dcKOs compared to controls (C-F,O).  Note that dcKOs still show expression of neurofilament (NFM) despite the reduction of MBP expression (E-F).  As observed in P7, Olig2 is reduced in dcKOs at P21 compared to controls (G-H,O) and Pdgfrα+ cell numbers remain similar between the different genotypes (I-J,O). At P21, Nkx2.2+ cells are similar in controls and dcKOs (K-L,O). To determine if mOL populations continue to improve with age, Plp1 was examined at P60 and remains reduced in dcKOs compared to controls (M-O). Quantification for respective stains counted in the corpus callosum (O). Scale bar in B and N = 1 mm (for A-B, M-N, respectively), D = 200 μm (C-F) and L = 100 μm (for G-L). CC= Corpus Callosum