Fig. 4

Tcf3/12 double conditional knock out mice show reduction of mature oligodendrocytes at postnatal time point P7. Tcf3/12 dcKOs have a dramatic reduction of mature oligodendrocytes, marked by Plp1 gene expression (A-B, I). Olig2, marking the entire OL lineage, is also decreased in the CC of dcKOs compared to controls (C-D, I). OPC marker, Pdgfrα, shows no difference in expression between controls and dcKOs, indicating a recovery from the E18.5 time point (E-F, I). Nkx2.2, expressed in differentiating OPCs, is also decreased at P7 in the dcKOs compared to controls (G-I). Images (C-H) are high magnification representatives of the corpus callosum in controls and dcKOs. Quantification of respective markers, counted in the corpus callosum (I). Scale bar in B = 1 mm (for A-B) and H = 100 μm (for C-H). CTX = Cortex, CC= Corpus Callosum, STM = Striatum