Fig. 2

E-protein expression throughout the oligodendrocyte lineage in the corpus callosum. Double immunofluorescence for Tcf4 (B, E, H, K), and Tcf12 (C, F, I, L) with OL lineage markers. β-gal was used as a reporter for Tcf3 in Tcf3^fx/+;FoxG1^IRES-Cre/+mice, where lacZ is recombined into frame under the Tcf3 promoter, and double stained for OL lineage markers (A, D, G, J). Olig2 was used to mark the entire OL lineage. All three E-proteins have some overlapping expression with Olig2 (see yellow arrows, A-C). Pdgfrα was used as a marker for OPC populations (see yellow arrows for overlap with E-proteins, D-F). Nkx2.2 marks maturing OPC populations (see yellow arrows for overlap, G-I). CC1 was used to define maturing OL populations (see yellow arrows for overlap, J-L). Quantification for percentage of OL lineage markers co-expressing the respective E-protein (M). Scale bar in L = 50 μm (for A-L)