Fig. 1

E-protein expression during telencephalon development. In situ hybridization for Tcf3 (A, D, G, J, M), Tcf4 (B, E, H, K, N), and Tcf12 (C, F, I, L, O). Representative images are shown at E13.5 (A-C), E15.5 (D-F), E18.5 (G-I), and P7 (J-O). At E13.5 and E15.5, all three E-proteins are enriched in the ventricular zone (VZ) in the CTX, LGE, and MGE (A-F). In addition, Tcf4 and Tcf12 also show high expression within the subventricular zone (SVZ) of the MGE at E13.5 and E15.5 (B-C, E-F). Enrichment for the E-proteins in the VZ continues at E18.5 and the SVZ at P7 (see black arrows G-L). Tcf4 specifically is highly enriched in the cortex at E15.5-P7 (see white asterisk, E, H, K). High magnification images of the corpus callosum at P7 reveal E-protein expression in the white matter track (M-O, black dashed box in J-L indicate the representative areas for M-O). Scale bar in F = 500 μm (for A-F), I = 600 μm (for G-I), L = 1 mm (for J-L), O = 200 μm (for M-O). LGE = lateral ganglionic eminence, MGE = medial ganglionic eminence, CTX = cortex, STM = striatum