Fig. 6
From: Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development
TrkB enrichment in the postsynaptic densities of DKO forebrain. Postsynaptic densities (PSD) were prepared from forebrains of E18.5 embryos. (a) Postnuclear supernatant (PNS), P2 (crude synaptosomes) and PSD fractions of DHET and DKO were analyzed by Western Blot analysis for Shank3, SAP102, PSD95, NR2B, TrkB and GluR1. Actin was used as loading control. Representative Western Blots were presented. (b) Ratios of SAP102, GluR1 and TrkB per actin were quantified for the PNS. N = 3 (SAP102 and TrkB) or 5 (GluR1), mean ± SD, ***: P < 0.001, *: P < 0.05, P > 0.05 not significant (ns) unpaired student’s t-test. (c) Ratio of PSD95, SAP102, Shank3 TrkB, GluR1 and NR2B per actin were quantified for PSD fraction. DHET were set to 100%. N = 3 (SAP102, TrkB and NR2B), 4 (PSD95) or 5 (GluR1 and Shank3), mean ± SD, **: P < 0.01 one-sample t-test