Fig. 2

DKO dendrites lack Golgi outposts. Hippocampal neurons were isolated at E18.5 and cultivated for 12 days in vitro (12 DIV). (a) DHET and DKO neurons were double-stained with primary antibodies against GM130 (red) and MAP2 (white). The nuclei were marked with Hoechst (blue). Confocal Z-stacks were taken and maximum intensity projections (MIP) were presented. (b) The percentage of cells with Golgi extensions into dendrites was determined for DHET and DKO as well as for Vti1a^−/− Vti1b^+/− and Vti1a^{+/ -} Vti1b^−/− at DIV12. N = 3–5 mean of at least 100 cells per experiment. Bars are mean of the mean of 4 different DHET, 5 DKO, 3 Vti1a^−/− Vti1b^+/− and 4 Vti1a^{+/ -} Vti1b^−/− embryos ± SEM. **: P < 0.01, ****: P < 0.0001 one-way ANOVA. (c) DKO neurons were transfected with GFP-Vti1a at 3 DIV and were further cultivated for 5 DIV. Cells were stained together with DHET and DKO (DIV8) as described above and dendrites were magnified. (d) A representative line scan through one dendrite per condition of GM130 signal is plotted over the length of the dendrite. Scale bars (a) 20 μm and (c) 10 μm