Fig. 3
From: Dpr10 and Nocte are required for Drosophila motor axon pathfinding
nocte mutants exhibit ISNb pathfinding errors. (A-D) m13 and m12 NMJs from respective genotypes. Neurons were visualized by staining for HRP (magenta) and the postsynapses were visualized by staining for DLG (green). Scale bar = 50 μm. (A, A′) Control w1118 animals with normal pathfinding (n = 90). Note that the ISNb travels above m13. (B, B′) nocteP animals (red) revealed pathfinding defects (n = 192). Here, MN12-Ib traveled in a different nerve (likely the TN) and m12 lacked innervation by vCE. (C, C′) nocte1 animals (cream) showed innervation defects (n = 102). Here, m12 was innervated from below m13 and m13 is innervated by only the vCE not the Ib NMJ. (D, D′) nocte-GAL4 x UAS-nocte-RNAix2 animals (cinnamon) also showed significant pathfinding defects (n = 96). Here, m12 and m13 are innervated after the nerve incorrectly traveled underneath m13. (E) Quantification of the frequency of pathfinding defects in the respective genotypes. Knockdown of nocte increased the frequency of ISNb pathfinding defects. *p < 0.05, **p < 0.01