Fig. 5

Immature neurons and neuro-precursor cells analysis. Thanks to our experimental design in which we gathered CNS at different stages of larval developpement, our atlas contains neurons at different stages along the differention axis neuroblast-functional neuron. a UMAP plot of the differentiating neurons. Neuroblasts and immature neurons at different stages of developpement cocluster sequentially along the first UMAP axis. The immature clusters occupy the lefmost part of the UMAP plot and are numbered as in Fig. 1b. Neuroblasts occupy the rightmost space and are numbered as in Fig. 1B and named after the presence of previously described markers. GMCs: Ganglion mother cells, INPs: Intermediate neural progenitors, TI NB: Type-1 neuroblasts, TII NB: Type-2 neuroblasts. b Pseudotime trajectory analysis of the developping neurons. To confirm the sequential co-clustering along the first UMAP axis we used the R package monocle3, to learn a pseudo-time trajectory in our developping neuron population. The trajectory was anchored at the cells with maximal Cyclin E (CycE) expression, and it’s depicted as a solid line starting at the INPs cluster. Cells are colored according to their pseudo-time value along the trajectory (see legend). Young cells (in pseudotime) coincide with neuroblast clusters while the oldest coincide with immature neuron clusters. c Immature neurons dotplot depicting the topmost enriched markers of each cluster. The numbering is the same as in Fig. 1b. d Neuroprecursor cells dotplot depicting the topmost enriched markers of each cluster. The numbering is the same as in Fig. 1b