Fig. 3

Connectome-consitent Kenyon Cell subtypes. Connectomic and developemental studies in Drosophila have established the temporal generation of morphologically distinct KCs during the larval stage. In agreement with this, the unsupervised clustering algorithm detected different KC clusters with different expression profiles. a UMAP plot of the Kenyon Cells clusters separated by stage. The cluster number corresponds to the numbering given in Fig. 1b. 1 h, 24 h, 48 h, refer to age in hours post hatching and prior to dissection. Each KC cluster comes from a different number of samples at each stage, the table shows the normalised the number of cells per stage at each stage. b Connectome KC cell types. Analysis of connectivity patterns among the larval KC population allowed their segregation in 3 morfological classes: 1/2-claw, 3 + -Claw and young. The matrix depicts the number of connections of all KC of the same and contralateral hemishpheres for each KC grouped by type. The intensity of blue of each matrix entry is proportional to the number of connections among 2 particular KCs. The bottom shows the volumetric reconstruction of the 3 classes of Kenyon Cells. c Dotplot depicting the topmost enriched KC markers compared to the other mature neuron clusters. KC: Kenyon Cells, Chol N: Cholinergic Neurons, Gaba N: Gabaergic Neurons, Glut N: Glutamatergic Neurons, Moto N: Motorneurons, Pept N: Peptidergic Neurons, Dopa N: Dopaminergic Neurons, Sero N: Serotoninergic Neurons, Octo N: Octopaminergic Neurons, Undet N: Undetermined Neurons. d Dotplot depicting the topmost enriched KC markers differentially expressed among the three KC clusters discovered in this study. KC: Kenyon Cells. Numbering as in Fig. 1b