Fig. 4
E15 sparse recombination induces significant morphological changes in bistratified RGCs of RetCreERt2 Brn3aCKOAP retinas. a-h represent example bistratified morphologies from the same experiments as shown in Fig. 2. RGCs were quantitated from RetCreERt2/WT; Brn3aCKOAP/WT and RetCreERt2/WT; Brn3aCKOAP/KO mice in which recombination was induced at E15 (10 and 8 mice, derived from 5 distinct experiments/litters), P0 (3 and 2 mice) or P22 (3 and 3 mice). The most frequently encountered bistratified RGC morphologies were ON–OFF DS (a-a3), SB (b-b3), Recursive (c-c3), Large bistratified (d-d3), ON-DS (e-e3), Brn3aKO-specific bistratified (f-f3), Abnormal type 1 (g-g4), and Abnormal type 2 (h-h4). For each cell focal planes through the cell body and axon (a, b, c, d, e, f, g, h), and the ON (a1, b1, c1, d1, e1, f1, g1-g2, h1) and OFF (a2, b2, c2, d2, e2, f2, g3, h2-h3) dendritic arbors are shown. Reconstructions, using the same conventions as in Fig. 2, are shown in (a3, b3, c3, d3, e3, f3, g4, h4). Scale bar in (h3) and horizontal bars delineating the IPL in reconstructions are 25 μm. Axons are green, vitreal (ON) dendrites are blue, scleral (OFF) dendrites are red, second (scleral) sub-layer of OFF dendrites in Abnormal bistratified type 2 cell is cyan (arrows in h3-h4). Recursive dendrites laminating in the ON arbor but branching from the OFF arbor, are labeled in yellow in b3, c3, d3 and h4 (arrowheads in b1 and b3, c1 and c3, d1 and d3, and h1 and h4). Morphological measurements for all bistratified RGCs are presented in Fig. 5