Fig. 4

UNC-34 directly binds to PPRs of WVE and Abi. A Cartoon representation of the WVE, Abi, and trimer constructs used in binding assays. PPR sequences predicted to bind to EVH1 domain are shown in the bottom right, with the binding motif in blue text. In ∆PPR constructs, the blue-colored sequences are replaced with the same number of amino acids containing “AGGGG”. B-E Coomassie-blue stained SDS PAGE gels showing GST-UNC-34 EVH1 pulling down purified WVE and Abi proteins. Images compare full-length or truncated proteins lacking the PPR sequences (B); full-length WVE and Abi carrying mutations that disrupt indicated PPR sequences (C); isolated PPR sequences (D); and various WVE/Abi/HSPC300 trimeric subcomplexes (E). Gel images are representative of at least two independent repeats