Fig. 2

Candidate enhancers ECR9 and ECR65 demonstrate specificity to ThrbCRM1(+) population and overlap with mitotic progenitors. (a) Overlap between enhancer::GFP expression (cyan) and ThrbCRM1::AU1 expression (magenta) 18–22 h after electroporation. DAPI is shown in the last column. Electroporated retina is found above the dotted line. Quantification of ThrbCRM1 reporter(+) cells in ECR9::GFP and ECR65::GFP cells. Percentages of ThrbCRM1(+) cells were calculated from a flow cytometry assay in which retinae were electroporated with ECR9::GFP or ECR65::GFP and ThrbCRM1::TdT and the number of GFP/ TdT double-positive cells out of the total GFP(+) population was calculated. (b) Overlap of endogenous Onecut1 expression (magenta) with ECR9::GFP and ECR65::GFP (cyan) 18–22 h after electroporation. Yellow arrows indicate cells that are GFP(+) and Onecut1(+). Scale bar in inset represents 25 um and applies to all insets. Percentages of Onecut1(+) cells were calculated by determining the number of Onecut1(+) and GFP(+) double-positive cells out of all enhancer::GFP(+) cells. (c) Overlap of 1 h EdU-pulsed cells with ECR9::GFP and ECR65::GFP (cyan) 18–22 h after electroporation. Yellow arrows indicate cells that are GFP(+) and EdU(+). Scale bar in inset represents 25 um and applies to all insets. Percentages of EdU(+) cells were calculated from confocal images by determining the number of EdU and enhancer::GFP double-positive cells out of all enhancer::GFP-positive cells. Each point represents a biological replicate with data collected from two images. (a, b, c) Each point represents a biological replicate. Error bars represent 95% confidence interval