Fig. 2
From: Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function
Ena is epistatic to Lar, Sdc, and Abl in NMJ growth. Gain of function (GOF) of the RPTP, Dlar (a), and associated HSPG ligand, Sdc (b), fail to rescue the bouton loss phenotype of UAS-FP4mito (enaLOF) flies when combined (DBL). Loss of the putative Ena suppressor Abl in Abl2/Df stE34 increases bouton number (c), supporting the antagonistic interaction of Ena and Abl. Full suppression of the Abl2/Df stE34 phenotype is observed in UAS-FP4mito (enaLOF) flies (c). Partial suppression of the Abl4/Df stE34 phenotype is observed with haplosufficient enaGC5/+ flies (d) indicating Ena is both downstream of and antagonized by Abl. Bouton number was determined by quantifying 1b and 1 s boutons. All results shown are statistically significant relative to control, with P < 0.05, as determined by Welch’s t-test. Error bars indicate ± s.e.m. of genotype; gray shading indicates ± s.e.m. of control; n ≥ 20 NMJs for all genotypes