Fig. 3

ECSF mice do not target myelinating Schwann cells. a Sciatic nerves of tamoxifen-injected ECSF; Ai14 mice were immunostained for myelinating Schwann cells (MBP, green) and nuclei (DAPI, blue). Lower row are enlarged views of the boxed area. A few myelin sheaths show TdTomato-expressing axons in the center (arrowheads). b Longitudinal view of the nerve shows TdTomato in axons. Representative images from three experiments are shown. Around 10.5% of axons were found to be targeted. Scales bars = 50 μm in upper row of a and in b; 10 μm in lower row of a. c Quantification of myelin sheaths with or without labelled axons shows 10.3% of axons are targeted in tamoxifen-injected mice. Three mice; 5 sections/mouse; error bar, standard error of mean