Fig. 5

Activation of the Lrig1+ neurogenic stem cells after Ara-C infusion. a A whole mount from a mouse infused Ara-C for 6 days. Scale bar, 10 μm. b A whole mount from a mouse infused Ara-C then chased for 1 day. Scale bar, 10 μm. c-f Even after the Ara-C infusion, some RFP+ cells did not enter the cell cycle. c-d α morphotype cells that had remained quiescent. Scale bar, 10 μm. e-f β morphotype cells that had remained quiescent. Scale bar, 10 μm. g However, some cells did enter the cell cycle. An α morphotype cell that entered the cell cycle as evident by positive Ki-67 immunoreactivity. Scale bar, 10 μm. h β morphotype cells that had entered the cell cycle, and apparently divided into two cells, also positive for Ki-67. Scale bar, 10 μm. i-j RFP+ cells clusters at 4 days after Ara-C infusion. The cells in clusters were immunoreactive for Ascl1 (i) or Dcx (j). Scale bar, 10 μm