Fig. 2
From: sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
Absence of sli results in mis-migrating and morphological irregularities in lch5 chordotonal neurons. a. WT stage 16 embryo labeled with anti-22C10 to mark all PNS sensory neurons showing the lch5 cluster in a lateral position in the embryo and the five lch5 neurons in an evenly-spaced diagonal row facing the dorsal-posterior edge of the embryo. b. Blown-up view of boxed region in A showing three abdominal hemisegments of PNS neurons. Black arrows point to lch5 neurons. c. sli mutant (sli2) embryo showing lch5 neurons either mis-migrating or displaying an irregular morphology. d. Blown-up view of boxed region in C showing three abdominal hemisegments of PNS neurons. Red arrows show lch5 neurons that have mis-migrated, and black arrowhead shows lch5 neurons with an irregular morphology. e. One hemisegment of a WT embryo labeled with anti-22C10 (red) which marks the cell membranes of all PNS neurons and anti-Elav (green) which stains the nuclei of all neurons. White arrow points to the lch5 neurons with normal morphology and the white arrowhead points to the v’ch1 neuron which is normally just ventral to the lch5 cluster. f. sli2 embryo showing lch5 neurons with irregular morphology (white arrow). The white arrowhead points to the v’ch1 neuron which is normally just ventral to the lch5 cluster. g. Quantification of percent total lch5 clusters displaying irregular morphology in WT, sli2, and a ubiquitous knock-down of sli using RNAi (Act5C-GAL4::UAS-sliRNAi) abbreviated as Act5C::sliRNAi. h. WT embryo showing lch5 cluster that has migrated to the correct lateral position. White arrow points to the position of the lch5 neurons and the white arrowhead points to the v’ch1 neuron which is normally just ventral to the lch5 cluster. i. sli2 embryo showing lch5 neurons that have not migrated ventrally (white arrow). The white arrowhead points to the v’ch1 neuron. The increased distance between the lch5 neurons and the v’ch1 neurons is visualized in the sli2 compared to the WT in h. j. Quantification of percent total lch5 mis-migrating clusters in WT, sli2, and Act5C::sliRNAi. Number of abdominal lch5 clusters scored for each genotype above each bar (n). **p < 0.05 based on a G-test of statistical independence