Fig. 7
Loss of Car8 does not delay mitotic progression in granule cell progenitors. (a) Schematic of tissue orientation and quantification location. (b) The number (no) of mitotically active cells in the P5 (n = 4) and P10 (n = 4) Car8wdl EGL is comparable to that in the P5 (n = 4) and P10 (n = 7) control EGL. P5, p = 0.5653; P10, p = 0.1773; Student’s t-test; Mean ± SEM. (c) The number (no) and density of PH3-positive cells in lobules V-VI of Car8wdl EGL (n = 4 at P5, 4 at P10) was not significantly different from that of lobules V-VI of control EGL (n = 4 at P5, 7 at P10). P5, p = 0.8940; P10, p = 0.1698; Student’s t-test; Mean ± SEM. The scale bar represents 50 μm. (d) Car8wdl (n = 4) granule cells progress through mitosis normally at P5 (n = 4 controls) and also at P10 (n = 4 mutants, n = 7 controls). P5 Prophase, p = 0.8287; P5 Prometaphase, p = 0.0608; P5 Metaphase, p = 0.2968; P5 Anaphase, p = 0.6297; P5 Telophase, p = 0.3989; P10 Prophase, p = 0.1452; P10 Prometaphase, p = 0.3282; P10 Metaphase, p = 0.2245; P10 Anaphase, p = 0.2421; P10 Telophase, p = 0.1594; Student’s t-test; Mean ± SEM. The scale bar represents 20 μm. (e) Loss of CAR8 does not affect the mitotic progression of granule cells, but does transiently delay their proliferation, relative to age-matched controls