Fig. 3

The neuroepithelial structure is severely impaired in the absence of Tcf7L2. a-d‘ Pax6⁺ RGC and Ctip2⁺ cortical neurons are reduced upon inactivation of Tcf7L2 alone (c-c“) or upon simultaneous deletion of Tcf7L1 and Tcf7L2 (d-d“). Inactivation of Tcf7L1 alone had a little influence on RGC and neuronal populations (b-b“). Panels in a‘ and a“ display magnified framed rectangles in a. e-g Quantifications of Pax6⁺, Ctip2⁺ and PCNA⁺ dividing cells showing an average from three independent experiments with standard deviations. Student’s paired t-test: *p < 0.05, **p < 0.01, ***p < 0.001 compared to controls, n = 8. h-k“ Double immunofluorescent labelling of PCNA⁺ dividing cells and ZO1⁺ apical adherens junctions in RGC. Dashed lines represent potential ventricular lining. Vertical arrows depict the thickness of the cortical layers II-IV containing Ctip2⁺ neurons