Fig. 3

Prdm13 overexpression promotes amacrine cells with a bias toward a glycinergic cell fate. a Illustration of the lipofection technique. i DNA is injected in the eye fields (green) of stage 18 embryos (frontal view). ii Retinas (green) are then sectioned (dashed line) at stage 41. iii Schema of a retinal section showing a clone of transfected cells (green) in the different retinal layers. iv Picture of a retinal section area (square in c) showing transfected cells (green). Nuclei are counterstained with Hoechst (blue). b Proportion of different retinal cell types in stage 41 prdm13 lipofected and control embryos. The table indicates the absolute numbers of counted cells for each cell type. c Double-immunostaining with anti-GFP and anti-Glycine or anti-GABA antibodies on retinal sections of prdm13 lipofected embryos. Arrows point to GFP positive cells that are Glycine or GABA-positive. d,e Quantification of GABA-positive and Glycine-positive cells among total GFP⁺ cells (d) or among GFP⁺ cells in the inner part of the INL where amacrine cells reside (e). Number of analysed retinas is indicated in each bar. GC: ganglion cells; AM: amacrine cells; BI: bipolar cells; HO: horizontal cells; PR: photoreceptor cells; MU: Müller cells. Values are given as mean ± SEM. * p-value <0,05; ** p-value <0,01; *** p-value <0,001 (Mann-Whitney test). Scale bar represents 50 μm