Fig. 2

Septum and LGE contribute to OB interneuron subtypes differently. (a) βgal⁺ (septum-derived) and tdTomato⁺ (LGE-derived) cells distributed around the P14 OB, despite their bias towards medial and lateral migration, respectively (inset of RMS (a’)). (b-e) βgal and tdTomato expressing cells were largely separated and showed distinct layer localization in the P14 OB. Boxes in (b) and (c) represent high magnification views in (d) and (e). βgal⁺ cells were enriched in the GL (b, d) whereas tdTomato⁺ cells were abundant in both GL and GCL (c-e). (f, g) LGE (tdTomato⁺) but not septum (βgal⁺) progenitors gave rise to CB⁺ PGCs. (h, i) TH⁺ PGCs did not originate from septum. Instead, many of them were generated from the LGE. (j, k) Both LGE (tdTomato⁺ cells indicated by arrows) and septum (βgal⁺ cells indicated by arrowheads) contributed to Sp8⁺ and CR⁺ PGCs. Boxes in (j) and (k) represent high magnification views in (j’, j”, k’, k”) respectively. (l) Quantification of each PGC subtypes from LGE- versus septum-lineages. Data represent the mean ± s.e.m.