Fig. 6

Normal overall expression of Islet1 in the vTel of Sema6a mutants and Plxna2; Plxna4 double mutants at E13.5. Double immunohistochemistry for neurofilament (green) and Islet1 (red) on E13.5 coronal brain sections indicates the preserved organization, at this stage, of Islet1-positive cell domains in the developing subpallium of Sema6a ^−/− (e, f), Plxna2 ^+/− ; Plxna4 ^+/− (c, d), Plxna2 ^−/− ; Plxna4 ^−/− (g, h) mouse brains, as compared to wild-type (a, b). Islet1-positive neurons are present in a narrow band situated immediately dorsal to extending TCAs, between the vTel subventricular zone and the globus pallidus (characterized by the absence of Islet1 immunostaining), and throughout the striatum, where neurofilament-expressing thalamocortical fibers can be observed to segregate (a, c, e, g). Caudally, a slight reduction and disorganization of the most posteriorly-located subset of Islet1-positive cells can be observed in the Plxna2 ^−/− ; Plxna4 ^−/− mouse vTel (h). Scale: 200 μm