Fig. 7
From: Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis
Kif20b mutant neurons have wider axons and minor neurites than controls. A Representative images of polarized control (+/-) and mutant (-/-) neurons cultured for two days and stained for actin (green), beta-III-tubulin (red) and nuclei (DAPI, blue). Insets show magnified axon segments for comparison. B Average width (±s.e.m.) of mutant axons (-/-, gray line) is greater than controls (+/-, black line) at each given distance from the edge of the soma. n = 79 +/- and 65 -/- axons. C Average width (±s.e.m.) of mutant minor neurites (gray line) is increased over controls (black line) at each given distance from the cell body. n = 323 +/- and 262 -/- minor neurites. D Representative images of and linescans across control (+/-) and mutant (+/-) axons stained for beta-III tubulin show that tubulin intensity was lower, more irregular, and more spread out in mutants than controls. Dotted lines represent where tubulin intensity linescans were taken. E Inset bar graph shows average total tubulin intensity summed across the width 25 μm from the soma was significantly decreased in mutant axons compared to controls. n = 15 each +/- and -/- from 1 experiment. *, p <.05; **, p <.01; ***, p <.001; ****, p <10-9. 10 μm scale bars