Fig. 6
From: Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis
Kif20b mutant neurons that polarize have shorter axons, longer minor neurites, and more axon branches. A Representative images of control (+/-) and mutant (-/-) polarized (Stage 3) neurons from E14.5 cortical cultures after 2 DIV, stained with phalloidin (actin, green), anti-beta-III-tubulin (red), and DAPI (blue). Blue arrows indicate axon branches. Red arrow indicates branch with only actin. Scale bar, 10 μm. B The average number of minor neurites (nascent dendrites) was slightly decreased in Kif20b mutant neurons (-/-, white bars), but was not statistically significant. C Individual minor neurites of Kif20b mutant neurons (-/-, white bars) are longer on average than controls’ at 2DIV. n = 323 +/-, 251 -/- minor neurites. D Summed dendritic length per cell is slightly increased in mutant neurons but is not statistically significant due to variable minor neurite number. E Kif20b mutant neurons (-/-, white bars) have shorter axons. n = 204 +/- and 260 -/- neurons. F Total axonal length including side branches was shorter in mutant neurons than controls. n = 202 +/- and 260 -/- neurons. G The average number of axon branches (containing tubulin) on mutant neurons was increased compared to controls. All length measurements were made based on tubulin staining not actin signal. For B, D and G, n = 79 +/-, 65 -/- neurons. Error bars are ± s.e.m. *, p <0.05; ***, p <0.001; ****, p <10-6; n.s., not significant