Fig. 2
From: Lacking of palladin leads to multiple cellular events changes which contribute to NTD
Cytoskeleton architecture is disturbed in Palladin -/- neural tube and cultured neural progenitors. (A) Cross-sections of embryos stained with FITC-conjugated Phalloidin and an antibody against P-cofilin, cultured neural progenitors stained with an antibody against Nestin and FITC-conjugated Phalloidin. The bundles of stress fibers in Palladin -/- neural tube (b, d) become obscure and faint compared with wt (a, c). Ratio of Nestin positive cells is over 98% in both wt and Palladin -/- neural progenitors (e, f). Stress fibers in Palladin -/- neural progenitors are atrophic (h) compared with wt (g) (B, C) mRNA levels of \( \alpha \) -actinin (B) and Argbp2 (C) are down-regulated in Palladin -/- embryo brains as assayed by qPCR. (D) \( \alpha \)-actinin is down-regulated in Palladin -/- embryo brains as assayed by western blotting. Magnifying power is 10X In A, e and f, while it is 20X in A, g and h. Error bars indicate SEM; *P < 0.05, ***P < 0.001