Fig. 2

TACC3 antagonizes Nocodazole-induced MT depolymerization but does not affect MT stability. a-c, Quantification of the MT dynamics shows significant reduction in MT growth speed (a), MT lifetime (b) and MT growth length (c) in control (n = 22) and TACC3 OE (n = 21) growth cones in response to 50 pM Nocodazole before and 5 min after drug treatment. However, the effect of Nocodazole on TACC3 OE growth cones is dampened compared to controls. a’-c’, Although not significant, reduction in MT growth speed (a’) is more prominent in control growth cones compared to TACC3 OE growth cones while the reduction in both lifetime (b’) and length (c’) in control growth cones are significantly higher than the TACC3 OE growth cones (d) Representative growth cone images of control, TACC3 KD and TACC3 OE, immunostained for tyrosinated tubulin (red) and detyrosinated tubulin (green) to label dynamic versus stable MTs, respectively. e-f, Quantification of the fluorescence intensity of imaging data in G, with TACC3 KD (n = 38) (e) and TACC3 OE (n = 129) (f) growth cones showing no significant changes in dynamic/stable MTs compared to corresponding control growth cones (n = 37 and n =143, respectively). *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant. n = growth cone number. Scale bar, 2 μm