Fig. 8
From: Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling
Slit and Robo mutants generate a normal oculomotor commissure. A-C Open book preparation of DiI and DiO back-labeled oculomotor nuclei. On E13.5, leading processes reached the contralateral nucleus. Neuron cell bodies, seen as bulges in the leading process, were in the midline (A, wild type control, Slit littermate). Loss of Slits or Robos (B, C) did not appear to reduce the number of leading processes or cells migrating through the floor plate. D-F. DiI back-label of the oculomotor nucleus on E16. 200 um coronal sections along the plane of the oculomotor nerve were compared by z-stacked confocal images. The oculomotor commissure was similar in thickness to wild type (D) in Slit and Robo mutants (E, F). (n = 3; control, Robo and Slit mutants) Scale bars, 100 μm