Fig. 6

Fissures form in a different order and over a shorter time window in En1 ^+/− ;En2 ^−/− mutants. a Midline sagittal section of an En2 ^+/− control animal. b Midline sagittal section of an En1 ^+/− ;En2 ^−/− mutant. Lobules in which the timing of gc production was quantified are color-coded (3 = AZ lobules in green, 7 = CZ lobule in blue, 8–9 = PZ lobules in purple, and 10 = NZ lobule in yellow). c Timing of formation of the fissures at the midline in controls (bottom) and En1 ^+/− ;En2 ^−/− mutants (top). Fissures are indicated in (a, b) by color-coded asterisks. The morphology of the anterior lobules in the mutant is variable (one to three fissures were present anterior to the posterior superior fissure, an example with 2 fissures is shown in (b) and the identity of the fissures could not be unequivocally determined, therefore the detection of an anterior fissure was recorded as precentral/preculminate/primary fissure (tricolored arrow)