Fig. 4

The kinetics of differentiation and proliferation are different in the anterior and central zones. a-b Portion of lobule 10 at P10 from sagittal midline sections are shown. a BrdU was administered 30 min before analysis marking cells in S-phase, Ki67 marks proliferating cells. b BrdU was administered 24 h before analysis, the cells that retain BrdU and are Ki67 negative are the cells that exited the cell cycle during the last 24 h. c Quantification of the level of proliferation as the percentage of Ki67 positive cells that are BrdU positive. d Quantification of the level of differentiation as the percentage of BrdU positive cell that are BrdU positive and Ki67 negative in the EGL. Quantifications were performed at P2, P6 and P10 in lobules 3 (AZ, green), 7 (CZ, blue) and 10 (NZ, yellow). p-values of the Dunnet’s post-hoc multiple comparisons tests following 2-way ANOVA comparing the levels of proliferation and differentiation between lobules at each time-point are shown when significant (see Additional file 1: Table S3)