Fig. 2
From: Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells
Depletion of DLK in Neuro-2a cells impairs neurite formation. a Representative phase contrast micrographs of control (pLKO.1) and DLK-depleted (sh73) Neuro-2a cells induced to differentiate for 24 h. Scale bar, 50 μm. b Neurite length of control and DLK-depleted Neuro-2a cells after differentiation. Values represent the mean length of neurites ± SEM (error bars) measured in five randomly chosen microscope fields for each sample (>200 neurites/experimental condition). Statistical significance was determined by unpaired Student’s t test. ****, p < 0.0001 compared with control cells. c Distribution of neurite lengths in control and DLK-depleted Neuro-2a cells after differentiation. Results are expressed as percentage of neurites ± SEM (error bars) with neurite length in the specified range. Statistical significance in neurite length between control and DLK-depleted cells was determined by the multiple t test using the Holm-Sidak method with α = 0.05 %. **, p < 0.01; ns, p > 0.05 vs control