Fig. 3

mir-135a-2 deletion by ZFN nuclease. a Representation of Rmst genomic structure. mir-135a-2 is indicated between exons 15 and 16. b Cartoon of the last intron of Rmst showing the location of mir-135a-2 and the ZFN cut site. Blue arrows show primers designed to detect the wild type and deleted bands. c Sequence of Rmst genomic region in which mir-135a-2 is embedded. Deleted region is underlined; PCR primers are indicated in blue; mir-135a-2 precursor sequence is in yellow; the ZFN binding site is in uppercases red and in lowercases is the ZFN cut site. d PCR showing the wild type and the ZFN deleted bands. e RT-qPCR showing a drastic reduction of miR-135a in E12.5 dissected dorsal forebrain tissue from control and mir-135a-2 null mice (n = 3). Data are shown as a fold change and have been normalized to microRNA sno202. ***, p value <0.001